Regionally varying F-actin network in the apical cytoplasm of ependymocytes

F-actin participates in morphogenetic cell-shape changes and helps maintain cellular integrity. Actin-like proteins have been detected in the ependymocytes of the cerebral ventricles, but the distribution of F-actin along the ventricular system has not been studied. We observed a highly ordered and regionally varying F-actin network in the apical cytoplasm of the ependyma in the ventricular system of rats using fluorescein isothiocyanate-conjugated phalloidin. Dense F-actin bundles spanned the entire circumference of the central canal of the spinal cord and formed a characteristic ring-like network in the apical region. The apical F-actin layer was widest in the lower cervical canal, and narrower in the upper thoracic canal. However, in the lower part of the filum terminale, the apical F-actin bundles became sparser and even disappeared. The apical F-actin layer differs significantly between the ventral and dorsal aspects above the medulla oblongata. This suggests that the regionally varying distribution of F-actin reflects the diverse local demands of the ependymocytes for cellular integrity and adhesive activity against external forces. (c) 2006 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.