Bistable secondary structures of small RNAs and their structural probing by comparative imino proton NMR spectroscopy

被引：54

作者：

Höbartner, C ^{[1
]}

Micura, R ^{[1
]}

机构：

[1] Univ Innsbruck, Inst Organ Chem, A-6020 Innsbruck, Austria

来源：

JOURNAL OF MOLECULAR BIOLOGY | 2003年 / 325卷 / 03期

基金：

奥地利科学基金会;

关键词：

RNA folding; secondary structure; conformational equilibria; multistable RNAs; imino protons;

D O I：

10.1016/S0022-2836(02)01243-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigate 25-34 nucleotide RNA sequences, that have been rationally designed to adopt two different secondary structures that are in thermodynamic equilibrium. Experimental evidence for the co-existence of the two conformers results from the NH...N H-1 NMR spectra. When compared to the NH...N H-1 NMR spectra of appropriate reference sequences the equilibrium position is easily quantifiable even without the assignment of the individual NH resonances. The reference sequences represent several Watson-Crick base-paired double helical segments, each encountered in either of the two conformers of the bistable target sequence. In addition, we rationalize the influence of nucleotide mutations on the equilibrium position of one of the bistable RNA sequences. The approach further allows a detailed thermodynamic analysis and the evaluation of secondary structure predictions for multistable RNAs obtained by computational methods. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：421 / 431

页数：11

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