Mobilization of lipid stores in Manduca sexta: cDNA cloning and developmental expression of fat body triglyceride lipase, TGL

被引:21
作者
Arrese, Estela L. [1 ]
Howard, Alisha D. [1 ]
Patel, Rajesh T. [1 ]
Rimoldi, Omar J. [1 ]
Soulages, Jose L. [1 ]
机构
[1] Oklahoma State Univ, Dept Biochem & Mol Biol, Stillwater, OK 74078 USA
基金
美国国家卫生研究院;
关键词
Lipase; TG lipase; TGL; AKH; Fat body; Lipolysis; Manduca sexta; Adipocytes; STORAGE DROPLET PROTEIN-1; HORMONE-INDUCED LIPOLYSIS; ADIPOKINETIC HORMONE; CARBOHYDRATE-METABOLISM; TOBACCO HORNWORM; TRIACYLGLYCEROL LIPASE; INSECT; IDENTIFICATION; ACTIVATION; STARVATION;
D O I
10.1016/j.ibmb.2009.12.008
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fatty acids stored as triglycerides (TG) in the fat body serve as precursor in multiple processes including energy production and synthesis of cellular components. Mobilization of fatty acids from TG depends on the action of lipases. The fat body triglyceride lipase from Manduca sexta, MsTGL, is the only insect lipase that has been purified and characterized, so far. A TGL cDNA from M. sexta fat body encoding a 649 amino acid protein was cloned and its identity confirmed by mass spectrometry and Edman sequencing data of the purified protein. The protein sequence has conserved domains and residues of potential importance for the function and regulation of TGL activity. The expression of TGL and the lipase activity of fat body homogenates were studied in several developmental stages of M. sexta. TC-hydrolase activity of fat body increased as larva grew to the last then, decreased to minimal levels during pupa stage. Lipase activity was progressively instar and, restored in adult insects and reached maximum values at this stage. The fat body lipase activity from adult insects, 1-2 day after emergence, was 9-fold higher than that from 2 to 3 days old 5th-instar larvae. A good correlation was found between the abundance of TGL protein and the lipase activity of fat body homogenates. This correlation and the expression pattern of TGL throughout development are consistent with the notion that TGL is the main fat body TG lipase of M. sexta. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码:91 / 99
页数:9
相关论文
共 41 条
[1]   The WWE domain: a common interaction module in protein ubiquitination and ADP ribosylation [J].
Aravind, L .
TRENDS IN BIOCHEMICAL SCIENCES, 2001, 26 (05) :273-275
[2]  
Arrese EL, 1997, J LIPID RES, V38, P68
[3]  
Arrese EL, 1999, J LIPID RES, V40, P556
[4]  
ARRESE EL, 1994, J LIPID RES, V35, P1652
[5]   Function and structure of lipid storage droplet protein 1 studied in lipoprotein complexes [J].
Arrese, Estela L. ;
Rivera, Laticia ;
Hamada, Masakazu ;
Mirza, Samna ;
Hartson, Steve D. ;
Weintraub, Susan ;
Soulages, Jose L. .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2008, 473 (01) :42-47
[6]   The main triglyceride-lipase from the insect fat body is an active phospholipase A1:: identification and characterization [J].
Arrese, Estela L. ;
Patel, Rajesh T. ;
Soulages, Jose L. .
JOURNAL OF LIPID RESEARCH, 2006, 47 (12) :2656-2667
[7]   Insect Fat Body: Energy, Metabolism, and Regulation [J].
Arrese, Estela L. ;
Soulages, Jose L. .
ANNUAL REVIEW OF ENTOMOLOGY, 2010, 55 :207-225
[8]   Expression of lipid storage droplet protein-1 may define the role of AKH as a lipid mobilizing hormone in Manduca sexta [J].
Arrese, Estela L. ;
Mirza, Saima ;
Rivera, Laticia ;
Howard, Alisha D. ;
Chetty, Palaniappan S. ;
Soulages, Jose L. .
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2008, 38 (11) :993-1000
[9]   Activation of triacylglycerol lipase in the fat body of a beetle by adipokinetic hormone [J].
Auerswald, L ;
Siegert, KJ ;
Gäde, G .
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2005, 35 (05) :461-470
[10]   Endocrine control of TAG lipase in the fat body of the migratory locust, Locusta migratoria [J].
Auerswald, Lutz ;
Gade, Gerd .
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2006, 36 (10) :759-768