Expression of vanilloid VR1 receptor in PC12 cells

被引:18
|
作者
Someya, A [1 ]
Kunieda, K [1 ]
Akiyama, N [1 ]
Hirabayashi, T [1 ]
Horie, S [1 ]
Murayama, T [1 ]
机构
[1] Chiba Univ, Grad Sch Pharmaceut Sci, Lab Chem Pharmacol, Chuo Ku, Chiba 2608675, Japan
关键词
vanilloid receptor 1; capsaicin; PC12; cells; intracellular Ca2+ concentrations;
D O I
10.1016/j.neuint.2004.06.002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Capsaicin, a pungent ingredient of chili pepper, activates vanilloid receptor subtype 1 (VR1), which is a nonselective cation channel with high Ca2+ permeability. Although VR1 and its splice variant are highly expressed in sensory neurons, they are expressed in neuronal cells in brain and peripheral non-neuronal cells. In this study, we investigated whether VR1 is expressed in PC12 cells, rat pheochromocytoma. Capsaicin at concentrations above 100 muM induced an increase in intracellular free Ca2+ concentrations by influx from extracellular spaces, and the effect was blocked by capsazepine, a selective antagonist of VR1. VR1 transcript and protein were detected by reverse transcription-polymerase chain reaction and Western blotting analysis, respectively. Immunocytochemical analysis revealed that VR1 protein was expressed in the cytosol and the plasma membrane of PC12 cells, and treatment with the antisense oligonucleotide for VR1 decreased the expression. VR1 in PC12 cells showed different characters from that in sensory neurons; capsaicin concentration-dependency and heat- and nerve growth factor-sensitivities. These results suggested that VR1 was functionally expressed in PC12 cells. The usefulness of PC12 cell line for studying functions and/or expression of VR1 is discussed. (C) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1005 / 1010
页数:6
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