The overlooked toxicity of environmentally persistent free radicals (EPFRs) induced by anthracene transformation to earthworms (Eisenia fetida)

被引：14
作者：
Zhu, Lang [1 ]
Liu, Jinbo [1 ]
Zhou, Jinyi [1 ]
Wu, Xintong [1 ]
Yang, Kangjie [1 ]
Ni, Zheng [1 ]
Liu, Ze [1 ]
Jia, Hanzhong [1 ,2 ]
机构：
[1] Northwest A&F Univ, Coll Nat Resources & Environm, Yangling 712100, Peoples R China
[2] Minist Agr & Rural Affairs, Key Lab Low Carbon Green Agr Northwestern China, Shanghai, Peoples R China
来源：
SCIENCE OF THE TOTAL ENVIRONMENT | 2022年 / 853卷
基金：
中国国家自然科学基金;
关键词：
Environmentally persistent free radicals; PAHs; Earthworm; Reactive oxygen species; Gut microbiota; POLYCYCLIC AROMATIC-HYDROCARBONS; INTEGRATED BIOMARKER RESPONSE; DNA-DAMAGE; LIPID-PEROXIDATION; PARTICULATE MATTER; AGRICULTURAL SOILS; OXIDATIVE STRESS; SYSTEM; DEGRADATION; PAHS;
D O I：
10.1016/j.scitotenv.2022.158571
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Environmentally persistent free radicals (EPFRs) as intermediate products exist widely in the PAHs-contaminated soils, but toxicity assessment associated with EPFRs for terrestrial invertebrates remains unclear. Using the model organism Eiseniafetida, we compared the adverse effects among anthracene (ANT), anthraquinone (ANQ), and EPFRs induced by ANT transformation on clay surfaces. Our results showed that EPFRs-exposed earthworms experienced histopatholo-gical damage, which was more severe than ANT and ANQ-exposed earthworms. The source of EPFRs damage was as-sociated with the obvious dysbiosis of reactive oxygen species in earthworms. Specifically, EPFRs trigged more severe antioxidant responses and oxidative damages (e.g., membrane lipid and DNA injury) in comparison with ANT and ANQ exposure, as evidenced by the values of integrated biomarker response (IBR) following the order of EPFRs (14.5) > ANT (12.8) > ANQ (10.9). Moreover, high-throughput sequencing found that EPFRs induced dramatic changes in the composition and structure of earthworm gut microbiota, which may involve immune and metabolism dysfunction, in turn aggravated EPFRs toxicity. Overall, the obtained information highlights the more severe injury of EPFRs to terrestrial organisms, deserving more attentions for the assessment of potential risks associated with radical intermediates in PAHs-contaminated soils.
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页数：11
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