DNA-modified artificial viral capsids self-assembled from DNA-conjugated -annulus peptide

被引：22

作者：

Nakamura, Yoko ^{[1
]}

Yamada, Saki ^{[1
]}

Nishikawa, Shoko ^{[1
]}

Matsuura, Kazunori ^{[1
]}

机构：

[1] Tottori Univ, Grad Sch Engn, Dept Chem & Biotechnol, Tottori 6808552, Japan

来源：

JOURNAL OF PEPTIDE SCIENCE | 2017年 / 23卷 / 7-8期

基金：

日本学术振兴会;

关键词：

self-assembly; DNA; -Annulus peptide; artificial viral capsid; ICOSAHEDRAL VIRUS-PARTICLES; ENCAPSULATION; DESIGN; NANOMATERIALS; PROTEINS; BINDING; CELLS;

D O I：

10.1002/psc.2967

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

beta-Annulus peptides from tomato bushy stunt virus conjugated with DNAs (dA(20) and dT(20)) at the C-terminal were synthesized. The DNA-modified -annulus peptides self-assembled into artificial viral capsids with sizes of 45-160nm. -Potential measurements revealed that the DNAs were coated on the surface of artificial viral capsids. Fluorescence assays indicated that the DNAs on the artificial viral capsids were partially hybridized with the complementary DNAs. Moreover, the DNA-modified artificial viral capsids formed aggregates by adding complementary polynucleotides. Copyright (c) 2017 European Peptide Society and John Wiley & Sons, Ltd.

引用

页码：636 / 643

页数：8

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