Gemini Surfactant Mediated Catansomes for Enhanced Singlet Oxygen Generation of Rose Bengal and Their Phototoxicity against Cancer Cells

被引:6
|
作者
Sharma, Bunty [1 ,2 ]
Samperi, Mario [2 ,5 ]
Jain, Akhil [6 ]
Chaudhary, Ganga Ram [1 ]
Kaur, Gurpreet [1 ]
Perez-Garcia, Lluisa [2 ,3 ,4 ]
机构
[1] Panjab Univ, Dept Chem, Ctr Adv Studies Chem, Chandigarh 160014, India
[2] Univ Nottingham, Sch Pharm, Div Adv Mat & Healthcare Technol, Nottingham NG7 2RD, England
[3] Univ Barcelona, Fac Farmacia & Ciencies Alimentacio, Dept Farmacol Toxicol & Quim Terapeut, Barcelona 08028, Spain
[4] Univ Barcelona, Inst Nanociencia Nanotecnol UB IN2UB, Barcelona 08028, Spain
[5] CNR, ITAE, Ist Tecnol Avanzate Energia Nicola Giordano, Messina, Italy
[6] Univ Nottingham, Sch Pharm, Div Regenerat Med & Cellular Therapies, Nottingham NG7 2RD, England
基金
英国工程与自然科学研究理事会;
关键词
Catansomes; Catanionic; Ethosomes; Vesicles; Singlet oxygen; Rose Bengal; Phototoxicity; PHOTODYNAMIC THERAPY; METALLOCATANIONIC VESICLES; IN-VITRO; CATANIONIC VESICLES; GOLD NANOPARTICLES; CELLULAR-UPTAKE; DELIVERY; PHOTOSENSITIZERS; EFFICIENT; SOLVENTS;
D O I
10.1021/acsbiomaterials.2c00017
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
Photodynamic therapy (PDT) is an innovativetechnique for cancer treatment with minimal side effects, based onthe use of a photosensitizer, oxygen, and light. Photosensitizers(PSs) have several limitations, that may limit their clinical use, likepoor solubilization, self-aggregation, and lack of specific targeting,which can be addressed with the use of nanomaterials. Herein, aunique type of catansomes (CaSs) was prepared using a geminiimidazolium-based surfactant (1,3-bis[(3-octadecyl-1-imidazolio)-methyl]benzene dibromide (GBIB) and a double chain surfactant,diaoctyl sodium sulfosuccinate or Aerosol OT (AOT). Theformation of CaS GBIB/AOT was optimized in various ethanol/water (E/W) solvent ratios by employing a facile, quick, and mostreliable solution-solution mixing method. The CaS was charac-terized by dynamic light scattering (DLS) andfield emission gun scanning electron microscopy (FEG-SEM) techniques. Theexperimental results reveal that stable CaSs with a spherical shape were obtained at lower concentration (100 mu M). Rose Bengal(RB), a PS of the xanthene family, was incorporated into these prepared CaSs, as proven byfluorescence spectroscopy, UV-visibleabsorption spectroscopy, and confocal laser scanning microscopy. Singlet oxygen (1O2) generation studies revealed the relevant roleof the E/W solvent ratio as there was a 4-fold boost in the1O2production for GBIB/AOT in E/W = 50:50 and around 3-fold in E/W = 30:70. Also, the GBIB-rich 80:20 fraction was more efficient in increasing the1O2generation as compared to the AOT richfraction (20:80). Further, their phototoxicity was tested in a water-rich solvent ratio (E/W = 30:70) against MCF-7 cells. Uponirradiation with a 532 nm laser (50 mW) for 5 min, RB@GBIB/AOT(20:80) fraction caused 50% decrease in the metabolic activityof MCF-7 cells, and RB@GBIB/AOT(80:20) fraction produced a maximum 85% decrease in cell viability. Furthermore, theenhancement in intracellular1O2generation by RB@GBIB/AOT, as compared to pure RB, was confirmed with singlet oxygensensor green (SOSG). This new type of CaS based on gemini surfactants exhibiting a large amount of1O2generation, holds greatinterest for several applications, such as use in photomedicine in future.
引用
收藏
页码:1878 / 1891
页数:14
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