A standard procedure for the micropropagation of the neem tree (Azadirachta indica A.!Juss)

被引:26
作者
Eeswara, JP
Stuchbury, T
Allan, EJ
Mordue, AJ
机构
[1] Univ Aberdeen, Dept Agr, Aberdeen AB24 5UA, Scotland
[2] Univ Aberdeen, Dept Zool, Aberdeen AB9 2TN, Scotland
关键词
neem; Azadirachta indica; micropropagation; shoot multiplication;
D O I
10.1007/s002990050381
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Micropropagated shoots were initiated from leaf explants of the neem tree, Azadirachta indica A. Juss. Regardless of their origin, shoots were successfully produced by culturing leaf explants on Murashige and Skoog medium containing benzylaminopurine (1 mg l(-1)), kinetin (0.8 mg l(-1)) and adenine sulphate (6 mg l(-1)) in complete darkness. These shoots were further multiplied on Murashige and Skoog medium containing benzylaminopurine (0.1 mg l(-1)), kinetin (0.08 g l(-1)) and adenine sulphate (0.6 mg l(-1)). Within 32 weeks, 80 shoots could be produced from a single leaf explant (10 mmx10 mm). Fifty-five percent of these shoots rooted on Murashige and Skoog medium containing indolebutyric acid (1 mg l(-1)) and all of these grew on transfer to soil.
引用
收藏
页码:215 / 219
页数:5
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