Protective effect of resveratrol on lens epithelial cell apoptosis in diabetic cataract rat

被引：15
作者：
Wang, Hong Min [1 ]
Li, Guo Xing [2 ]
Zheng, Han Song [1 ]
Wu, Xue Zhi [1 ]
机构：
[1] Zhengzhou Univ, Affiliated Hosp Ctr, Zhumadian 463000, Peoples R China
[2] Eye Hosp Affiliated, Wenzhou Med Coll, Wenzhou 325000, Peoples R China
来源：
ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE | 2015年 / 8卷 / 02期
关键词：
Resveratrol; Diabetic cataract; Lens epithelial cell; Cell apoptosis; GENE-EXPRESSION; MITOCHONDRIAL; PGC-1-ALPHA;
D O I：
10.1016/S1995-7645(14)60307-2
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
Objective: To study the protective effect of resveratrol on lens epithelial cell apoptosis in diabetic cataract rat. Methods: A total of 84 Wistar rats were divided into 4 groups: 12 in Group A (control group), 24 in Group B (diabetic cataract group), 24 in Group C (therapeutic dose of resveratrol group) and 24 in Group D low-dose of resveratrol group). Rats in Group B-D were given with 60 mg/kg streptozotocin through intraperitoneal injection. Rats in Group C were given with 100 mg/kg resveratrol and rats in Group D were given with 20 mg/kg resveratrol. The caspase-3 expression levels and apoptosis ratios of LEG among each group were observed: the degrees of lens opacity in Group B-D after 12 weeks were compared. Results: There were significant differences in caspase-3 expression levels, apoptosis ratios of LEC among groups at 4 w, 8 w and 12 w (P<0.05). After 12 weeks, in Group B the degree of lens opacity was as follow: 0 (0.00%) in grade I, 3 (37.50%) in grade II,2 (25.00%)in grade III, 2 (25.00%)grade IV. and 1(12.50%) in grade V: in Group C: 2 (25.00%)in grade, 4 50.00%) in grade 11 2 (25.00%)in grade III, 0 (0.00%)grade IV, and 0 (0.00 %) in grade V; in Croup D: 1 (12.50%)in grade I, 4 (50.00%) in grade II. 2 (25.00%) in grade III, 1 (12.50%) grade IV, and 0 (0.00%) in grade V. The difference among Group B-D was statistically significant (P<0.05). Conclusions: Resveratrol has protective effect on lens epithelial cell apoptosis in diabetic cataract rat, and the effect is relative to its dose.
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页码：153 / 156
页数：4
相关论文
共 16 条
  • [1] Chandrasena L. G., 2008, Southeast Asian Journal of Tropical Medicine and Public Health, V39, P731
  • [2] Chen XF, 2013, J HARBIN MED U, V47, P229
  • [3] Hong L, 2013, CARBOHYD POLYM, V92, P1211, DOI DOI 10.1016/j.carbpol.2012.10.037
  • [4] Jia Songbai, 2012, Zhong Nan Da Xue Xue Bao Yi Xue Ban, V37, P730, DOI 10.3969/j.issn.1672-7347.2012.07.015
  • [5] Adipose-derived mesenchymal stem cells ameliorate STZ-induced pancreas damage in type 1 diabetes
    Li, Yue-Ying
    Liu, Han-Han
    Chen, Hong-Lei
    Li, Yin-Ping
    [J]. BIO-MEDICAL MATERIALS AND ENGINEERING, 2012, 22 (1-3) : 97 - 103
  • [6] The overexpression of SIRT1 inhibited osteoarthritic gene expression changes induced by interleukin-1β in human chondrocytes
    Matsushita, Takehiko
    Sasaki, Hiroshi
    Takayama, Koji
    Ishida, Kazunari
    Matsumoto, Tomoyuki
    Kubo, Seiji
    Matsuzaki, Tokio
    Nishida, Kotaro
    Kurosaka, Masahiro
    Kuroda, Ryosuke
    [J]. JOURNAL OF ORTHOPAEDIC RESEARCH, 2013, 31 (04) : 531 - 537
  • [7] HOMEOSTASIS MODEL ASSESSMENT - INSULIN RESISTANCE AND BETA-CELL FUNCTION FROM FASTING PLASMA-GLUCOSE AND INSULIN CONCENTRATIONS IN MAN
    MATTHEWS, DR
    HOSKER, JP
    RUDENSKI, AS
    NAYLOR, BA
    TREACHER, DF
    TURNER, RC
    [J]. DIABETOLOGIA, 1985, 28 (07) : 412 - 419
  • [8] Nonautonomous Apoptosis Is Triggered by Local Cell Cycle Progression during Epithelial Replacement in Drosophila
    Nakajima, Yu-ichiro
    Kuranaga, Erina
    Sugimura, Kaoru
    Miyawaki, Atsushi
    Miura, Masayuki
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 2011, 31 (12) : 2499 - 2512
  • [9] PGC-1α is coupled to HIF-1α-dependent gene expression by increasing mitochondrial oxygen consumption in skeletal muscle cells
    O'Hagan, Kathleen A.
    Cocchiglia, Sinead
    Zhdanov, Alexander V.
    Tambawala, Murtaza M.
    Cummins, Eoin P.
    Monfared, Mona
    Agbor, Terence A.
    Garvey, John F.
    Papkovsky, Dmitri B.
    Taylor, Cormac T.
    Allan, Bernard B.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (07) : 2188 - 2193
  • [10] Diabetic cataracts: mechanisms and management
    Obrosova, Irina G.
    Chung, Stephen S. M.
    Kador, Peter F.
    [J]. DIABETES-METABOLISM RESEARCH AND REVIEWS, 2010, 26 (03) : 172 - 180
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