Myosin-IIA heavy chain phosphorylation on S1943 regulates tumor metastasis

被引：14

作者：

Toro, Laura E. Norwood ^{[1
]}

Wang, Yarong ^{[2
]}

Condeelis, John S. ^{[2
,6
,7
]}

Jones, Joan G. ^{[2
,4
,5
,6
]}

Backer, Jonathan M. ^{[1
,3
]}

Bresnick, Anne R. ^{[1
]}

机构：

[1] Albert Einstein Coll Med, Dept Biochem, 1300 Morris Pk Ave, Bronx, NY 10461 USA

[2] Albert Einstein Coll Med, Dept Anat & Struct Biol, 1300 Morris Pk Ave, Bronx, NY 10461 USA

[3] Albert Einstein Coll Med, Dept Mol Pharmacol, 1300 Morris Pk Ave, Bronx, NY 10461 USA

[4] Albert Einstein Coll Med, Dept Pathol, 1300 Morris Pk Ave, Bronx, NY 10461 USA

[5] Albert Einstein Coll Med, Dept Epidemiol & Populat Hlth, 1300 Morris Pk Ave, Bronx, NY 10461 USA

[6] Albert Einstein Coll Med, Integrated Imaging Program, 1300 Morris Pk Ave, Bronx, NY 10461 USA

[7] Albert Einstein Coll Med, Gruss Lipper Biophoton Ctr, 1300 Morris Pk Ave, Bronx, NY 10461 USA

来源：

EXPERIMENTAL CELL RESEARCH | 2018年 / 370卷 / 02期

关键词：

Myosin-II; Phosphorylation; Invasion; Matrix degradation; Invadopodia; BREAST-CANCER; MATRIX DEGRADATION; CELL INVASION; PODOSOME DYNAMICS; CARCINOMA-CELLS; IN-VIVO; INVADOPODIA; MATURATION; S100A4; MATRIX-METALLOPROTEINASE-9;

D O I：

10.1016/j.yexcr.2018.06.028

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Nonmuscle myosin-IIA (NMHC-IIA) heavy chain phosphorylation has gained recognition as an important feature of myosin-II regulation. In previous work, we showed that phosphorylation on 51943 promotes myosin-IIA filament disassembly in vitro and enhances EGF-stimulated lamellipod extension of breast tumor cells. However, the contribution of NMHC-IIA 51943 phosphorylation to the modulation of invasive cellular behavior and metastasis has not been examined. Stable expression of phosphomimetic (S1943E) or non-phosphorylatable (S1943A) NMHC-IIA in breast cancer cells revealed that 51943 phosphorylation enhances invadopodia function, and is critical for matrix degradation in vitro and experimental metastasis in vivo. These studies demonstrate a novel link between NMHC-IIA 51943 phosphorylation, the regulation of extracellular matrix degradation and tumor cell invasion and metastasis.

引用

页码：273 / 282

页数：10

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