Saliva is a reliable and accessible source for the detection of SARS-CoV-2

被引：47

作者：

Herrera, Luis A. ^{[1
,2
]}

Hidalgo-Miranda, Alfredo ^{[3
]}

Reynoso-Noveron, Nancy ^{[2
]}

Meneses-Garcia, Abelardo A. ^{[4
]}

Mendoza-Vargas, Alfredo ^{[5
]}

Reyes-Grajeda, Juan P. ^{[5
]}

Vadillo-Ortega, Felipe ^{[6
]}

Cedro-Tanda, Alberto ^{[1
]}

Penaloza, Fernando ^{[2
]}

Frias-Jimenez, Emmanuel ^{[1
]}

Arriaga-Canon, Cristian ^{[2
]}

Ruiz, Rosaura ^{[7
]}

Angulo, Ofelia ^{[7
]}

Lopez-Villasenor, Imelda ^{[8
]}

Amador-Bedolla, Carlos ^{[9
]}

Vilar-Compte, Diana ^{[4
]}

Cornejo, Patricia ^{[4
]}

Cisneros-Villanueva, Mireya ^{[3
]}

Hurtado-Cordova, Eduardo ^{[3
]}

Cendejas-Orozco, Mariana ^{[3
]}

Hernandez-Morales, Jose S. ^{[5
]}

Moreno, Bernardo ^{[2
]}

Hernandez-Cruz, Irwin A. ^{[2
]}

Herrera, Cesar A. ^{[2
]}

Garcia, Francisco ^{[2
]}

Gonzalez-Woge, Miguel A. ^{[2
]}

Munguia-Garza, Paulina ^{[2
]}

Luna-Maldonado, Fernando ^{[2
]}

Sanchez-Vizcarra, Antonia ^{[2
]}

Osnaya, Vincent G. ^{[2
]}

Medina-Molotla, Nelly ^{[5
]}

Alfaro-Mora, Yair ^{[2
]}

Caceres-Gutierrez, Rodrigo E. ^{[2
]}

Tolentino-Garcia, Laura ^{[2
]}

Rosas-Escobar, Patricia ^{[5
]}

Roman-Gonzalez, Sergio A. ^{[1
]}

Escobar-Arrazola, Marco A. ^{[2
]}

Canseco-Mendez, Julio C. ^{[5
]}

Ortiz-Soriano, Diana R. ^{[1
]}

Dominguez-Ortiz, Julieta ^{[2
]}

Gonzalez-Barrera, Ana D. ^{[1
]}

Aparicio-Bautista, Diana, I ^{[1
]}

Cruz-Rangel, Armando ^{[1
]}

Alarcon-Zendejas, Ana Paula ^{[2
]}

Contreras-Espinosa, Laura ^{[2
]}

Gonzalez, Rodrigo ^{[2
]}

Guerra-Calderas, Lissania ^{[2
]}

Meraz-Rodriguez, Marco A. ^{[2
]}

Montalvo-Casimiro, Michel ^{[2
]}

Montiel-Manriquez, Rogelio ^{[2
]}

机构：

[1] Inst Nacl Med Genom, INMEGEN, Mexico City, DF, Mexico

[2] Univ Nacl Autonoma Mexico, Inst Nacl Cancerol, Inst Invest Biomed, Unidad Invest Biomed Canc, Mexico City, DF, Mexico

[3] Inst Nacl Med Genom, Lab Genom Canc, INMEGEN, Mexico City, DF, Mexico

[4] Inst Nacl Cancerol, INCAN, Unidad Secuenciac, Mexico City, DF, Mexico

[5] Inst Nacl Med Genom, INMEGEN, Unidad Secuenciac, Mexico City, DF, Mexico

[6] INMEGEN, Unidad Vinculac Cient Fac Med, Mexico City, DF, Mexico

[7] Secretaria Educ Ciencia Tecnol & Innovat, Mexico City, DF, Mexico

[8] Univ Nacl Autonoma Mexico, Inst Invest Biomed, Mexico City, DF, Mexico

[9] Univ Nacl Autonoma Mexico, Fac Quim, Dept Fis & Quim Teor, Mexico City, DF, Mexico

[10] Unidad Temporal COVID 19, Mexico City, DF, Mexico

[11] Unidad Invest Preclin, Fac Quim, Mexico City, DF, Mexico

[12] Univ Nacl Autonoma Mexico, Fac Quim, Dept Alimentos & Biotecnol, Mexico City, DF, Mexico

[13] Inst Diagnost & Referencia Epidemiol, InDRE, Mexico City, DF, Mexico

来源：

INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES | 2021年 / 105卷

关键词：

COVID-19; SARS-CoV-2; Diagnostic test; Saliva testing; Pooling strategy;

D O I：

10.1016/j.ijid.2021.02.009

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Objectives: The aim of this study was to investigate the feasibility of saliva sampling as a non-invasive and safer tool to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and to compare its reproducibility and sensitivity with nasopharyngeal swab samples (NPS). The use of sample pools was also investigated. Methods: A total of 2107 paired samples were collected from asymptomatic healthcare and office workers in Mexico City. Sixty of these samples were also analyzed in two other independent laboratories for concordance analysis. Sample processing and analysis of virus genetic material were performed according to standard protocols described elsewhere. A pooling analysis was performed by analyzing the saliva pool and the individual pool components. Results: The concordance between NPS and saliva results was 95.2% (kappa 0.727, p = 0.0001) and 97.9% without considering inconclusive results (kappa 0.852, p = 0.0001). Saliva had a lower number of inconclusive results than NPS (0.9% vs 1.9%). Furthermore, saliva showed a significantly higher concentration of both total RNA and viral copies than NPS. Comparison of our results with those of the other two laboratories showed 100% and 97% concordance. Saliva samples are stable without the use of any preservative, and a positive SARS-CoV-2 sample can be detected 5, 10, and 15 days after collection when the sample is stored at 4 degrees C. Conclusions: The study results indicate that saliva is as effective as NPS for the identification of SARS-CoV-2-infected asymptomatic patients. Sample pooling facilitates the analysis of a larger number of samples, with the benefit of cost reduction. (C) 2021 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.

引用

页码：83 / 90

页数：8

共 32 条

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