A 5-Enolpyruvylshikimate 3-Phosphate Synthase Functions as a Transcriptional Repressor in Populus

被引：64

作者：

Xie, Meng ^{[1
,2
]}

Muchero, Wellington ^{[1
,2
]}

Bryan, Anthony C. ^{[1
,2
]}

Yee, Kelsey ^{[1
,2
]}

Guo, Hao-Bo ^{[3
]}

Zhang, Jin ^{[1
,2
]}

Tschaplinski, Timothy J. ^{[1
,2
]}

Singan, Vasanth R. ^{[4
]}

Lindquist, Erika ^{[4
]}

Payyavula, Raja S. ^{[1
,2
]}

Barros-Rios, Jaime ^{[5
,6
]}

Dixon, Richard ^{[5
,6
]}

Engle, Nancy ^{[1
,2
]}

Sykes, Robert W. ^{[7
]}

Davis, Mark ^{[7
]}

Jawdy, Sara S. ^{[1
,2
]}

Gunter, Lee E. ^{[1
,2
]}

Thompson, Olivia ^{[1
,2
]}

DiFazio, Stephen P. ^{[8
]}

Evans, Luke M. ^{[8
]}

Winkeler, Kim ^{[9
]}

Collins, Cassandra ^{[9
]}

Schmutz, Jeremy ^{[4
,10
]}

Guo, Hong ^{[3
]}

Kalluri, Udaya ^{[1
,2
]}

Rodriguez, Miguel ^{[1
,2
]}

Feng, Kai ^{[1
,2
]}

Chen, Jin-Gui ^{[1
,2
]}

Tuskan, Gerald A. ^{[1
,2
,4
]}

机构：

[1] Oak Ridge Natl Lab, BioEnergy Sci Ctr, Oak Ridge, TN 37831 USA

[2] Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN 37831 USA

[3] Univ Tennessee, Dept Biochem & Cellular & Mol Biol, Knoxville, TN 37996 USA

[4] USDA, Joint Genome Inst, Walnut Creek, CA 94598 USA

[5] Univ North Texas, BioDiscovery Inst, Denton, TX 76203 USA

[6] Univ North Texas, Dept Biol Sci, Denton, TX 76203 USA

[7] Natl Renewable Energy Lab, Biosci Ctr, Golden, CO 80401 USA

[8] West Virginia Univ, Dept Biol, Morgantown, WV 26506 USA

[9] ArborGen, Ridgeville, SC 29472 USA

[10] HudsonAlpha Inst Biotechnol, Huntsville, AL 35806 USA

来源：

PLANT CELL | 2018年 / 30卷 / 07期

关键词：

GENE-EXPRESSION; NATURAL-POPULATIONS; WALL BIOSYNTHESIS; SHIKIMATE PATHWAY; SECONDARY; GENOME; XYLEM; EVOLUTION; SEQUENCE; POPLAR;

D O I：

10.1105/tpc.18.00168

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Long-lived perennial plants, with distinctive habits of inter-annual growth, defense, and physiology, are of great economic and ecological importance. However, some biological mechanisms resulting from genome duplication and functional divergence of genes in these systems remain poorly studied. Here, we discovered an association between a poplar (Populus trichocarpa) 5-enolpyruvylshikimate 3-phosphate synthase gene (PtrEPSP) and lignin biosynthesis. Functional characterization of PtrEPSP revealed that this isoform possesses a helix-turn-helix motif in the N terminus and can function as a transcriptional repressor that regulates expression of genes in the phenylpropanoid pathway in addition to performing its canonical biosynthesis function in the shikimate pathway. We demonstrated that this isoform can localize in the nucleus and specifically binds to the promoter and represses the expression of a SLEEPER-like transcriptional regulator, which itself specifically binds to the promoter and represses the expression of PtrMYB021 (known as MYB46 in Arabidopsis thaliana), a master regulator of the phenylpropanoid pathway and lignin biosynthesis. Analyses of overexpression and RNAi lines targeting PtrEPSP confirmed the predicted changes in PtrMYB021 expression patterns. These results demonstrate that PtrEPSP in its regulatory form and PtrhAT form a transcriptional hierarchy regulating phenylpropanoid pathway and lignin biosynthesis in Populus.

引用

页码：1645 / 1660

页数：16

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