Engineering Polymerases for New Functions

被引：31

作者：

Coulther, Timothy A. ^{[1
]}

Stern, Hannah R. ^{[1
]}

Beuning, Penny J. ^{[1
]}

机构：

[1] Northeastern Univ, Dept Chem & Chem Biol, Boston, MA 02115 USA

来源：

TRENDS IN BIOTECHNOLOGY | 2019年 / 37卷 / 10期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

AQUATICUS DNA-POLYMERASE; HOT START; FAMILY; PCR; AMPLIFICATION; REPLICATION; MUTATIONS; EVOLUTION; PROCESSIVITY; RESISTANCE;

D O I：

10.1016/j.tibtech.2019.03.011

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

DNA polymerases are critical tools in biotechnology, enabling efficient and accurate amplification of DNA templates, yet many desired functions are not readily available in natural DNA polymerases. New or improved functions can be engineered in DNA polymerases by mutagenesis or through the creation of protein chimeras. Engineering often necessitates the development of new techniques, such as selections in water-in-oil emulsions that connect genotype to phenotype and allow more flexibility in engineering than phage display. Engineering efforts have led to DNA polymerases that can withstand extreme conditions or the presence of inhibitors, as well as polymerases with the ability to copy modified DNA templates. In this review we discuss polymerases for biotechnology that have been reported along with tools to enable further development.

引用

页码：1091 / 1103

页数：13

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