Humic acids isolated from earthworm compost enhance root elongation, lateral root emergence, and plasma membrane H+-ATPase activity in maize roots

被引:473
作者
Canellas, LP
Olivares, FL
Okorokova-Façanha, AL
Façanha, AR
机构
[1] Univ Estadual Norte Fluminense, Lab Biol Celular & Tecidual, Ctr Biociencias Biotecnol, BR-28015620 Rio De Janeiro, Brazil
[2] Univ Estadual Norte Fluminense, Lab Solos, Ctr Ciencias Tecnol Agropecuarias, BR-28015620 Rio De Janeiro, Brazil
[3] Univ Estadual Norte Fluminense, Lab Fisiol Bioquim Microrganismos, BR-28015620 Rio De Janeiro, Brazil
关键词
D O I
10.1104/pp.007088
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Earthworms (Eisenia foetida) produce humic substances that can influence plant growth by mechanisms that are not yet clear. In this work, we investigated the effects of humic acids (HAs) isolated from cattle manure earthworm compost on the earliest stages of lateral root development and on the plasma membrane H+-ATPase activity. These HAs enhance the root growth of maize (Zea mays) seedlings in conjunction with a marked proliferation of sites of lateral root emergence. They also stimulate the plasma membrane H+-ATPase activity, apparently associated with an ability to promote expression of this enzyme. In addition, structural analysis reveals the presence of exchangeable auxin groups in the macrostructure of the earthworm compost HA. These results may shed light on the hormonal activity that has been postulated for these humic substances.
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页码:1951 / 1957
页数:7
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