A myosin I isoform in the nucleus

被引：201
作者：
Pestic-Dragovich, L
Stojiljkovic, L
Philimonenko, AA
Nowak, G
Ke, Y
Settlage, RE
Shabanowitz, J
Hunt, DF
Hozak, P
de Lanerolle, P [1 ]
机构：
[1] Univ Illinois, Dept Physiol & Biophys, Chicago, IL 60612 USA
[2] Acad Sci Czech Republ, Inst Expt Med, Dept Cell Ultrastruct & Mol Biol, Prague, Czech Republic
[3] Univ Virginia, Dept Chem, Charlottesville, VA 22901 USA
[4] Univ Virginia, Dept Pathol, Charlottesville, VA 22901 USA
来源：
SCIENCE | 2000年 / 290卷 / 5490期
关键词：
D O I：
10.1126/science.290.5490.337
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A nuclear isoform of myosin I beta that contains a unique 16-amino acid aminoterminal extension has been identified. An affinity-purified antibody to the 16-amino acid peptide demonstrated nuclear staining. Confocal and electron microscopy revealed that nuclear myosin I beta colocalized with RNA polymerase II in an alpha-amanitin- and actinomycin D-sensitive manner. The antibody coimmunoprecipitated RNA polymerase II and blocked in vitro RNA synthesis. This isoform of myosin I beta appears to be in a complex with RNA polymerase II and may affect transcription.
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收藏
页码：337 / 341
页数：5
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