CD54+ rabbit adipose-derived stem cells overexpressing HIF-1α facilitate vascularized fat flap regeneration

被引:7
作者
Li, De-Quan [1 ]
Lu, Guan-Ming [2 ]
Liang, Yi-Dan [3 ]
Liang, Zhi-Jie [4 ]
Huang, Min-Hong [4 ]
Peng, Qi-Liu [3 ]
Zou, Dong-Hua [5 ]
Gu, Rong-He [6 ]
Xu, Fang-Tian [7 ]
Gao, Hui [7 ]
Chen, Zhen-Dong [7 ]
Chi, Guang-Yi [8 ]
Wei, Zhong-Heng [2 ]
Chen, Li [9 ]
Li, Hong-Mian [8 ]
机构
[1] Third Hosp Nanchang City, Dept Mammary Glands Surg, Nanchang 330009, Jiangxi, Peoples R China
[2] Youjiang Med Univ Nationalities, Affiliated Hosp, Dept Glands Surg, Baise 533000, Peoples R China
[3] Guangxi Med Univ, Affiliated Hosp 5, Cent Lab Med Sci, Nanning 530022, Peoples R China
[4] Guangxi Med Univ, Affiliated Hosp 5, Dept Mammary Glands Surg, Nanning 530022, Peoples R China
[5] Guangxi Med Univ, Affiliated Hosp 5, Dept Neurol, Nanning 530022, Peoples R China
[6] Guangxi Med Univ, Affiliated Hosp 5, Dept Orthoped Surg, Nanning 530022, Peoples R China
[7] Gannan Med Univ, Affiliated Hosp 1, Dept Orthoped, Ganzhou 341000, Peoples R China
[8] Guangxi Med Univ, Affiliated Hosp 5, Dept Plast & Aesthet Surg, Nanning 530022, Peoples R China
[9] Guangxi Med Univ, Affiliated Hosp 1, Dept Neurol, Nanning 530021, Peoples R China
关键词
rabbit adipose-derived stem cells; HIF-1; alpha; adipogenic differentiation; engineered fat flaps; angiogenesis; PLATELET-RICH PLASMA; SOFT-TISSUE REGENERATION; HYPOXIA-INDUCIBLE FACTOR-1; ADIPOGENIC DIFFERENTIATION; STROMAL CELLS; FACTOR-I; DEPENDENT MECHANISM; ENDOTHELIAL-CELLS; ANGIOGENESIS; GRAFTS;
D O I
10.18632/oncotarget.16777
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Fat flap transplantation is frequently performed in patients suffering from soft tissue defects resulting from disease or trauma. This study explored the feasibility of constructing vascularized fat flaps using rabbit adipose-derived stem cells (rASCs) and collagen scaffolds in a rabbit model. We evaluated rASCs proliferation, paracrine function, adipogenesis, vascularization, and CD54 expression, with or without HIF-1 alpha transfection in vitro and in vivo. We observed that adipogenic differentiation potential was greater in rASCs with high CD54 expression (CD54(+)rASCs) than in those with low expression (CD54(-)rASCs), both in vitro and in vivo. HIF-1 alpha overexpression not only augmented this effect, but also enhanced cell proliferation and paracrine function in vitro. We also demonstrated that HIF-1 alpha-transfected CD54(+)rASCs showed enhanced paracrine function and adipogenic capacity, and that paracrine function increases expression of angiogenesis-related markers. Thus, CD54(+)rASCs overexpressing HIF-1 alpha enhanced large volume vascularized fat flap regeneration in rabbits, suggesting CD54 may be an ideal candidate marker for ASCs adipogenic differentiation.
引用
收藏
页码:46875 / 46890
页数:16
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