One-pot conversion of levan prepared from Serratia levanicum NN to difructose anhydride IV by Arthrobacter nicotinovorans levan fructotransferase

被引：9
|
作者：
Kikuchi, Hiroto [2 ]
Sakurai, Hiroaki [2 ]
Nagura, Taizo [2 ]
Aritsuka, Tsutomu [2 ]
Tomita, Fusao [3 ]
Yokota, Atsushi [1 ]
机构：
[1] Hokkaido Univ, Res Fac Agr, Lab Microbial Physiol, Kita Ku, Sapporo, Hokkaido 0608589, Japan
[2] Nippon Beet Sugar Mfg Co Ltd, Res Ctr, Obihiro, Hokkaido 0800831, Japan
[3] Univ Air, Hokkaido Study Ctr, Kita Ku, Sapporo, Hokkaido 0600817, Japan
来源：
JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2010年 / 109卷 / 03期
关键词：
beta-D-fructofuranose-beta-D-fructofuranose-2,6 ':6,2 '-dianhydride DFA IV; Levan; Sucrose; Levan fructotransferase; Arthrobacter nicotinovorans; Serratia levanicum; DFA IV; CALCIUM-ABSORPTION; PURIFICATION; GS-9; GENE;
D O I：
10.1016/j.jbiosc.2009.09.041
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The newly established difructose anhydride IV (DFA IV) production system is comprised of the effective production of levan from sucrose by Serratia levanicum NN, the conversion of the levan into DFA IV by levan fructotransferase from Arthrobacter nicotinovorans GS-9, which is highly expressed in an Escherichia coli transformant, and a practical purification step. The chemical properties of DFA IV were also investigated. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.
引用
收藏
页码：240 / 243
页数：4
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