Enhanced production of xylanase by a newly isolated Aspergillus terreus under solid state fermentation using palm industrial waste: A statistical optimization

被引:71
作者
Lakshmi, Garapati Suvarna [1 ]
Rao, Chaganti Subba [1 ]
Rao, Ravella Sreenivas [2 ]
Hobbs, Phil J. [2 ]
Prakasham, Reddy Shetty [1 ]
机构
[1] Indian Inst Chem Technol, Bioengn & Environm Ctr, Hyderabad 500607, Andhra Pradesh, India
[2] N Wyke Res, Okehampton EX20 2SB, Devon, England
基金
英国生物技术与生命科学研究理事会;
关键词
Xylanase; Hemicellulosic material hydrolyzing enzyme; Aspergillus terreus; Optimization; Solid state fermentation; Taguchi methodology; ALKALINE PROTEASE PRODUCTION; ISOLATED BACILLUS-CIRCULANS; XYLITOL PRODUCTION; WHEAT-STRAW; HIGH-LEVEL; CELLULASE; TOLERANT; ENZYME; BRAN;
D O I
10.1016/j.bej.2009.08.005
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Xylanase production by a newly isolated Aspergillus terreus MTCC 8661 was optimized using palm fiber in solid state fermentation (SSF). Different fermentation parameters such as Incubation temperature. moisture content, medium pH, particle size, incubation time. inoculum, xylose and sodium nitrate concentrations were investigated at the individual and interactive level by the Taguchi methodology All selected fermentation parameters influenced xylanase production Moisture content, Incubation time and inoculum concentration were the major [similar to 85%] influential parameters on xylanase production at the individual level. At the interactive level. inoculum concentration was important and accounted for more than 50% of (fie severity index with particle size and Incubation temperature Xylanase production improved from 41,000 to 115,000 U/g indicating 227% improvement after optimization suggesting that this fungal strain, A terreus MTCC 8661, has the commercial potential for hemicellulosic enzyme production (C) 2009 Published by Elsevier B V
引用
收藏
页码:51 / 57
页数:7
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