Evaluation of a rapid immunochromatographic test for detection of KPC in clinical isolates of Enterobacteriaceae and Pseudomonas species

被引：16

作者：

Wozniak, Aniela ^{[1
,2
]}

Paillavil, Braulio ^{[1
]}

Legarraga, Paulette ^{[1
,2
]}

Zumaran, Cecilia ^{[2
]}

Prado, Sandra ^{[2
]}

Garcia, Patricia ^{[1
,2
]}

机构：

[1] Pontificia Univ Catolica Chile, Dept Clin Labs, Escuela Med, Santiago 7820436, Chile

[2] Red Salud UC CHRISTUS, Lab Microbiol, Santiago, Chile

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2019年 / 95卷 / 02期

关键词：

KPC; Carbapenemase detection; Immunochromatographic test; Pseudomonas species;

D O I：

10.1016/j.diagmicrobio.2019.05.009

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The KPC K-SeT immunochromatographic test (Coris BioConcept (R), Gembloux, Belgium) has been widely used for detection of KPC in Enterobacteriaceae with reported sensitivities and specificities of 100%. However, to our knowledge, there are no reports of its use in KPC-positive Pseudomonas species. We evaluated the KPC K-SeT test in 36 clinical isolates of Enterobacteriaceae (21 KPC-positive and 15 KPC-negative) and 20 Pseudomonas species (5 KPC-positive and 15 KPC-negative) using conventional PCR for carbapenemase genes as the reference method. The KPC K-SeT test detected 25 out of 26 KPC-positive isolates (96.1%). The undetected isolate was 1 P. aeruginosa bearing the mutation D179Y in the omega loop region of KPC-2 carbapenemase. This mutation was already reported in Enterobacteriaceae as conferring resistance to ceftazidime-avibactam. To our knowledge, this is the first report of evaluation of KPC K-SeT test in KPC-positive P. aeruginosa isolates. (C) 2019 Elsevier Inc. All rights reserved.

引用

页码：131 / 133

页数：3

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