Site-specific functionalisation of proteins by a Staudinger-type reaction using unsymmetrical phosphites

被引：33

作者：

Boehrsch, Verena ^{[1
,2
]}

Serwa, Remigiusz ^{[1
]}

Majkut, Paul ^{[1
]}

Krause, Eberhard ^{[3
]}

Hackenberger, Christian P. R. ^{[1
]}

机构：

[1] Free Univ Berlin, Inst Chem & Biochem, D-14195 Berlin, Germany

[2] Beuth Hsch Tech Berlin, Fachbereich Life Sci & Technol, Biochem Lab, D-13347 Berlin, Germany

[3] Leibniz Inst Mol Pharmacol, D-13125 Berlin, Germany

来源：

CHEMICAL COMMUNICATIONS | 2010年 / 46卷 / 18期

关键词：

COPPER-FREE; RECOMBINANT PROTEINS; LIVING CELLS; CHEMISTRY; AZIDES; STRATEGIES; PHOSPHORYLATION; CYCLOADDITIONS; SELECTIVITY; PHOSPHORUS;

D O I：

10.1039/b926818a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Unsymmetrical phosphites react efficiently in a Staudinger reaction with p-azido-phenylalanine, which can be applied for the biotinylation of proteins, thereby expanding the scope of the chemoselective Staudinger-phosphite reaction of aryl azides with symmetrical phosphites to the corresponding phosphoramidates.

引用

页码：3176 / 3178

页数：3

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