Tepidopterin, 1-O-(L-threo-biopterin-2′-yl)-β-N-acetylglucosamine from Chlorobium tepidum

被引:45
作者
Cho, SH
Na, JU
Youn, H
Hwang, CS
Lee, CH
Kang, SO [1 ]
机构
[1] Seoul Natl Univ, Coll Nat Sci, Dept Microbiol, Biophys Lab, Seoul 151742, South Korea
[2] Seoul Natl Univ, Res Ctr Mol Microbiol, Seoul 151742, South Korea
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 1998年 / 1379卷 / 01期
关键词
tepidopterin; L-threo-biopterin; N-acetylglucosamine; (Chlorobium tepidum);
D O I
10.1016/S0304-4165(97)00081-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A novel pterin compound, designated as tepidopterin, was detected from a thermophilic photosynthetic green sulphur bacterium, Chlorobium tepidum. The amount of tepidopterin inside the cell was estimated to be 2-5 mu mol g(-1) dry weight of cell. This compound was purified through a high performance Liquid chromatography using preparative DeltaPak C-18 column. This compound was characterized by chromatographic behavior and by absorption and fluorescence properties. Its structure was determined to be 1-O-(L-threo-biopterin-2'-yl)-beta-N-acetylglucosamine by 1D- and 2D-NMR spectroscopy, mass spectrometry and CD. The relative amount of tetrahydrotepidopterin was estimated to be 96.7% inside the cell, that of dihydrotepidopterin 2.9%, and that of fully oxidized tepidopterin 0.4%. The amount of tepidopterin within the cell increased continuously until the beginning of the stationary phase of the cell growth. (C) 1998 Elsevier Science B.V.
引用
收藏
页码:53 / 60
页数:8
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