Insight into the Rhizomucor miehei lipase supported on chitosan-chitin nanowhiskers assisted esterification of eugenol to eugenyl benzoate

被引:31
作者
Abd Manan, Fatin Myra [1 ]
Attan, Nursyafreena [1 ]
Zakaria, Zainoha [1 ]
Mahat, Naji Arafat [1 ]
Wahab, Roswanira Abdul [1 ]
机构
[1] Univ Teknol Malaysia, Fac Sci, Dept Chem, Johor Baharu 81310, Malaysia
关键词
Chitosan; Immobilized Rhizomucor miehei lipase; Chitin nanowhiskers; Nano-filler; Eugenyl benzoate; CANDIDA-RUGOSA LIPASE; WALLED CARBON NANOTUBES; SUSTAINABLE PRODUCTION; ENZYMATIC-SYNTHESIS; GERANYL PROPIONATE; IMMOBILIZED LIPASE; METHYL OLEATE; ACID; ADSORPTION; STABILITY;
D O I
10.1016/j.jbiotec.2018.05.015
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
To overcome drawbacks in the conventional chemical route to synthesize eugenyl benzoate, immobilized Rhizomucor miehei lipase (RML) as the biocatalyst was proposed. The RML conjugated to a hybrid support consisting of biopolymers, chitosan (CS) and chitin nanowhiskers (CNWs). 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDAC) was used as the crosslinker to bind the lipase. Immobilization of RML was the highest on crosslinked CS/CNWs which gave a protein loading of similar to 8.12 mg/g, corresponding to specific and residual activity of 537 U/g and 137%, respectively. Fourier transform infrared spectroscopy, thermogravimetric analysis-differential thermogravimetry, field emission scanning electron and atomic force microscopy of RMLCS/CNWs revealed that RML was successfully attached to the surface of crosslinked CS/CNWs. Under an optimized condition, the highest yield of eugenyl benzoate (56.3%) was attained after 5 h using 3 mg/mL of RMLCS/CNWs with molar ratio of eugenol: benzoic acid of 3:1, as compared to only 47.3% for the free RML. Analyses of FTIR and NMR on purified eugenyl benzoate affirmed that the ester was successfully produced in the enzymatic esterification. Therefore, the use of the RML-CS/CNWs biocatalysts appears promising to afford good yields of eugenyl benzoate within a relatively shorter reaction time.
引用
收藏
页码:19 / 30
页数:12
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