Effect of poly-glutamate on uptake efficiency and cytotoxicity of cell penetrating peptides

被引：11

作者：

Farkhani, Samad Mussa ^{[1
,2
,3
]}

Shirani, Ali ^{[1
,2
,3
]}

Mohammadi, Samaneh ^{[1
,2
,3
]}

Zakeri-Milani, Parvin ^{[4
,5
]}

Mojarrad, Javid Shahbazi ^{[5
,6
]}

Valizadeh, Hadi ^{[5
,7
]}

机构：

[1] Tabriz Univ Med Sci, Fac Adv Med Sci, Res Ctr Pharmaceut Nanotechnol, Tabriz, Iran

[2] Tabriz Univ Med Sci, Dept Med Nanotechnol, Fac Adv Med Sci, Tabriz, Iran

[3] Tabriz Univ Med Sci, Student Res Comm, Tabriz, Iran

[4] Tabriz Univ Med Sci, Liver & Gastrointestinal Dis Res Ctr, Tabriz, Iran

[5] Tabriz Univ Med Sci, Fac Pharm, Tabriz, Iran

[6] Tabriz Univ Med Sci, Biotechnol Res Ctr, Tabriz, Iran

[7] Tabriz Univ Med Sci, Drug Appl Res Ctr, Tabriz, Iran

来源：

IET NANOBIOTECHNOLOGY | 2016年 / 10卷 / 02期

关键词：

cellular biophysics; polymers; drug delivery systems; biomedical materials; toxicology; molecular biophysics; drug delivery; A549; cell; plasma membrane; pore formation; cancer cells; poly-glutamate; cytotoxicity; uptake efficiency; cell penetrating peptides; ARGININE-RICH PEPTIDES; DRUG-DELIVERY; PLASMA-MEMBRANE; UPTAKE KINETICS; INTERNALIZATION; PROTEOGLYCANS; MECHANISM; TOXICITY; ACID;

D O I：

10.1049/iet-nbt.2015.0030

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cell penetrating peptides (CPPs) were developed as vehicles for efficient delivery of various molecules. An ideal CPP-peptide should not display any toxicity against cancer cells as well as healthy cells and efficiently enter into the cell. Because of the cationic nature and the intrinsic vector capabilities, these peptides can cause cytotoxicity. One of the possible reasons for toxicity of CPPs is direct translocation and consequently, pore formation on the plasma membrane. In this study it was demonstrated that interaction of poly-glutamate with CPP considerably reduced their cytotoxicity in A549 cell. This strategy could be useful for efficient drug delivery mediated by CPP.

引用

页码：87 / 95

页数：9

共 40 条

[1] Systemic in vivo distribution of activatable cell penetrating peptides is superior to that of cell penetrating peptides [J].