Immunohistochemical Study of Rabies Virus Within the Central Nervous System of Domestic and Wildlife Species

被引:61
作者
Stein, L. T. [1 ]
Rech, R. R. [1 ]
Harrison, L. [1 ]
Brown, C. C. [1 ]
机构
[1] Univ Georgia, Coll Vet Med, Dept Pathol, Athens, GA 30602 USA
关键词
central nervous system; immunohistochemistry; lyssavirus; rabies; viral encephalitis; FLUORESCENT-ANTIBODY TEST; FIXED BRAIN-TISSUE; LABORATORY DIAGNOSIS; VIRAL-ANTIGEN;
D O I
10.1177/0300985810370013
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Immunohistochemistry using a commercial polyclonal antibody for lyssavirus was applied to 39 archival cases of rabies. Paraffin blocks from 13 different species were available, including 3 dogs, 4 cats, 1 pig, 6 cattle, 4 horses, 1 llama, 7 skunks (Mephitis mephitis), 7 raccoons (Procyon lotor), 1 bat (Myotis species), 1 white-tailed deer (Odocoileus virginianus), 1 bobcat (Lynx rufus), 2 gray foxes (Urocyon cinereoargenteus), and 1 red fox (Vulpes vulpes). All cases had previously been diagnosed as rabies using histopathology and/or fluorescent antibody testing. The immunohistochemistry technique successfully detected lyssavirus antigen in all cases. In species for which 3 or more samples were available, distributional trends were seen in 4 main brain regions: brainstem, cerebellum, hippocampus, and cerebrum. The best site for rabies virus detection in dogs and cats was the hippocampus. For cattle, viral antigen was most prominent in the brainstem, followed by the cerebellum. In horses, the cervical spinal cord and adjacent brainstem were the optimal sites for detecting rabies virus antigen. In raccoons and skunks, positive labeling was widely dispersed, so selection might be less important for these wildlife reservoir species. Immunohistochemistry should prove useful in enhancing the accuracy of rabies diagnosis through informed selection of brain sampling sites when composite sampling is not feasible. This immunohistochemical technique could provide reliable virus detection in formalin-fixed tissues in any potentially infected species.
引用
收藏
页码:630 / 636
页数:7
相关论文
共 27 条
[1]  
[Anonymous], 2004, INFECT DIS LIVESTOCK
[2]  
[Anonymous], EQUINE INFECT DIS
[3]   Detection of rabies viral antigens in non-autolysed and autolysed tissues by using an immunoperoxidase technique [J].
Arslan, A ;
Saglam, YS ;
Temur, A .
VETERINARY RECORD, 2004, 155 (18) :550-552
[4]   Distribution of rabies antigen in infected brain material: determining the reliability of different regions of the brain for the rabies fluorescent antibody test [J].
Bingham, J ;
van der Merwe, M .
JOURNAL OF VIROLOGICAL METHODS, 2002, 101 (1-2) :85-94
[5]   Laboratory diagnosis of equine rabies and its implications for human postexposure prophylaxis [J].
Carrieri, Maria Luiza ;
Pinheiro Peixoto, Zia Maria ;
Barradas Paciencia, Mateus Luis ;
Kotait, Ivanete ;
Leal Germano, Pedro Manuel .
JOURNAL OF VIROLOGICAL METHODS, 2006, 138 (1-2) :1-9
[6]  
GREEN SL, 1992, J AM VET MED ASSOC, V200, P1133
[7]   Comparative Pathological Study of the Murine Brain after Experimental Infection with Classical Rabies Virus and European Bat Lyssaviruses [J].
Hicks, D. J. ;
Nunez, A. ;
Healy, D. M. ;
Brookes, S. M. ;
Johnson, N. ;
Fooks, A. R. .
JOURNAL OF COMPARATIVE PATHOLOGY, 2009, 140 (2-3) :113-126
[8]  
Jackson A.C., 2007, RABIES, V2nd
[9]   Immunohistochemical study of human rabies [J].
Jogai, S ;
Radotra, BD ;
Banerjee, AK .
NEUROPATHOLOGY, 2000, 20 (03) :197-203
[10]   Rabies among infrequently reported mammalian carnivores in the United States, 1960-2000 [J].
Krebs, JW ;
Williams, SM ;
Smith, JS ;
Rupprecht, CE ;
Childs, JE .
JOURNAL OF WILDLIFE DISEASES, 2003, 39 (02) :253-261