Sulfated heterorhamnans from the green seaweed Gayralia oxysperma: partial depolymerization, chemical structure and antitumor activity

被引：37

作者：

Ropellato, Juliana ^{[1
]}

Carvalho, Mariana M. ^{[1
]}

Ferreira, Luciana G. ^{[1
]}

Noseda, Miguel D. ^{[1
]}

Zuconelli, Cristiane R. ^{[2
]}

Goncalves, Alan G. ^{[3
]}

Ducatti, Diogo R. B. ^{[1
]}

Kenski, Juliana C. N. ^{[2
]}

Nasato, Pauline. L. ^{[1
]}

Winnischofer, Sheila M. B. ^{[2
]}

Duarte, Maria E. R. ^{[1
]}

机构：

[1] Univ Fed Parana, Dept Bioquim & Biol Mol, Lab Glicobiol Estrutural Carboidratos Algas Marin, BR-81531980 Curitiba, Parana, Brazil

[2] Univ Fed Parana, Dept Bioquim & Biol Mol, Lab Cult Celular, BR-81531980 Curitiba, Parana, Brazil

[3] Univ Fed Parana, Dept Farm, BR-81531980 Curitiba, Parana, Brazil

来源：

CARBOHYDRATE POLYMERS | 2015年 / 117卷

关键词：

Sulfated polysaccharides; Partial depolymerization; Chemical structure; Antitumor activity; Gayralia oxysperma; Green seaweed; RHAMNAN SULFATE; ANTICOAGULANT ACTIVITY; POLYSACCHARIDES; MANNANS; ALGAE;

D O I：

10.1016/j.carbpol.2014.09.089

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Sulfated heterorhamnans produced by Gayralia oxysperma were utilized for the preparation of two homogeneous and highly sulfated Smith-degraded products (Mwof 109 and 251 kDa), which were constituted principally by 3-linked alpha-L-rhamnosyl units 2- or 4-sulfate and 2-linked alpha-L-rhamnosyl units 4- or 3,4-sulfate, in different percentages. The homogeneous products and the crude extracts containing the sulfated heterorhamnans showed cytotoxic effect against U87MG cells. These sulfated polysaccharides induced an increase in the number of cells in G1 phase with concomitant increase of the mRNA levels of p53 and p21. The presence of 2-linked disulfated rhamnose residues together with the molecular weight could be important factors to be correlated with the inhibitory effect on human glioblastoma cells. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：476 / 485

页数：10

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