Label-Free Biosensor Arrays Based on Silicon Ring Resonators and High-Speed Optical Scanning Instrumentation

被引：452

作者：

Iqbal, Muzammil ^{[1
]}

Gleeson, Martin A. ^{[1
]}

Spaugh, Bradley ^{[1
]}

Tybor, Frank ^{[1
]}

Gunn, William G. ^{[1
]}

Hochberg, Michael ^{[2
]}

Baehr-Jones, Tom ^{[2
]}

Bailey, Ryan C. ^{[3
]}

Gunn, L. Cary ^{[1
]}

机构：

[1] Genalyte Inc, San Diego, CA 92121 USA

[2] Univ Washington, Dept Elect Engn, Seattle, WA 98195 USA

[3] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

来源：

IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS | 2010年 / 16卷 / 03期

基金：

美国国家卫生研究院;

关键词：

Refractometric sensor; resonant cavity sensors; ring resonator sensor; optical biosensing; MICRORING-RESONATOR; SENSOR; SURFACE;

D O I：

10.1109/JSTQE.2009.2032510

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

A platform for performing rapid, real-time binding assays on sensor arrays based on silicon ring resonators is presented in this paper. An array of 32 sensors is interrogated simultaneously. Using eight sensors as controls, 24 simultaneous binding curves are produced. The bulk refractive index sensitivity of the system was demonstrated down to 7.6 x 10(-7) and sensor-to-sensor variability is 3.9%. Using an 8-min incubation, real-time binding was observed over 8-logs of concentration down to 60 fM using immobilized biotin to capture streptavidin diluted in bovine serum albumin solution. Multiplexing in complex media is demonstrated with two DNA oligonucleotide probes. Time to result and repeatability are demonstrated to be adequate for clinical applications.

引用

页码：654 / 661

页数：8

共 29 条

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