Analysis of rpsL and rrs mutations in Beijing and non-Beijing streptomycin-resistant Mycobacterium tuberculosis isolates from Singapore

被引：46

作者：

Sun, Y-J. ^{[2
]}

Luo, J-T. ^{[1
]}

Wong, S-Y. ^{[2
]}

Lee, A. S. G. ^{[1
]}

机构：

[1] Natl Canc Ctr, Div Med Sci, Singapore 169610, Singapore

[2] Tan Tock Seng Hosp, Dept Infect Dis, Singapore, Singapore

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2010年 / 16卷 / 03期

基金：

英国医学研究理事会;

关键词：

Beijing genotype; Mycobacterium tuberculosis; rpsL mutations; rrs mutations; streptomycin resistance; DRUG-RESISTANT; GENOTYPE; STRAINS; RUSSIA; GENE;

D O I：

10.1111/j.1469-0691.2009.02800.x

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

P>The Beijing genotype of Mycobacterium tuberculosis has frequently been found to be associated with drug resistance. Mutation analysis of the genes encoding 16S rRNA (rrs) and ribosomal protein S12 (rpsL) revealed a high frequency (97/102; 95.1%) of alterations in streptomycin-resistant M. tuberculosis isolates from Singapore, with rpsL K43R being the most common rpsL mutation (82/92; 89%), which was significantly associated with Beijing strains compared to non-Beijing strains (odds ratio = 10.88, 95% confidence interval = 3.48-34.1). This is the first study to report the association of Beijing strains with the rpsL K43R mutation in STR-resistant M. tuberculosis isolates with de novo resistance, as determined by clustering analysis.

引用

页码：287 / 289

页数：3

共 20 条

[1]

Anh DD, 2000, EMERG INFECT DIS, V6, P302

[2] Silent nucleotide polymorphisms and a phyogeny for Mycobacterium tuberculosis [J].

Baker, L ;

Brown, T ;

Maiden, MC ;

Drobniewski, F .

EMERGING INFECTIOUS DISEASES, 2004, 10 (09) :1568-1577

[3] Geographic variation of the predictive values of genomic mutations associated with streptomycin resistance in Mycobacterium tuberculosis [J].

Dobner, P ;

Bretzel, G ;

RuschGerdes, S ;

Feldmann, K ;

Rifai, M ;

Loscher, T ;

Rinder, H .

MOLECULAR AND CELLULAR PROBES, 1997, 11 (02) :123-126

[4] Drug-resistant, tuberculosis, clinical virulence, and the dominance of the Beijing strain family in Russia [J].

Drobniewski, F ;

Balabanova, Y ;

Nikolayevsky, V ;

Ruddy, M ;

Kuznetzov, S ;

Zakharova, S ;

Melemyev, A ;

Fedorin, I .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2005, 293 (22) :2726-2731

[5] MOLECULAR-BASIS OF STREPTOMYCIN RESISTANCE IN MYCOBACTERIUM-TUBERCULOSIS - ALTERATIONS OF THE RIBOSOMAL-PROTEIN S12 GENE AND POINT MUTATIONS WITHIN A FUNCTIONAL 16S RIBOSOMAL-RNA PSEUDOKNOT [J].

FINKEN, M ;

KIRSCHNER, P ;

MEIER, A ;

WREDE, A ;

BOTTGER, EC .

MOLECULAR MICROBIOLOGY, 1993, 9 (06) :1239-1246

[6] Disequilibrium in distribution of resistance mutations among Mycobacterium tuberculosis Beijing and non-beijing strains isolated from patients in Germany [J].

Hillemann, D ;

Kubica, T ;

Rüsch-Gerdes, S ;

Niemann, S .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2005, 49 (03) :1229-1231

[7] STREPTOMYCIN RESISTANCE IN MYCOBACTERIA [J].

HONORE, N ;

COLE, ST .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1994, 38 (02) :238-242

[8] Characterization of the rpsL and rrs genes of streptomycin-resistant clinical isolates of Mycobacterium tuberculosis in Japan [J].

Katsukawa, E ;

Tamaru, A ;

Miyata, Y ;

Abe, C ;

Makino, M ;

Suzuki, Y .

JOURNAL OF APPLIED MICROBIOLOGY, 1997, 83 (05) :634-640

[9]

Lee Ann S. G., 2002, International Journal of Infectious Diseases, V6, P48, DOI 10.1016/S1201-9712(02)90136-0

[10] Contribution of kasA analysis to detection of isoniazid-resistant Mycobacterium tuberculosis in Singapore [J].

Lee, ASG ;

Lim, IHK ;

Tang, LLH ;

Telenti, A ;

Wong, SY .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (08) :2087-2089

← 1 2 →