Facile preparation of glyconanoparticles and their bioconjugation to streptavidin

被引:71
作者
Housni, Abdelghani
Cai, Haijun
Liu, Shiyong
Pun, Suzie H.
Narain, Ravin [1 ]
机构
[1] Laurentian Univ, Dept Chem & Biochem, Biomol Sci Program, Peterborough PE3 2C6, England
[2] Univ Sci & Technol China, Dept Polymer Sci & Engn, Hefei, Anhui, Peoples R China
[3] Univ Washington, Dept Bioengn, Seattle, WA 98195 USA
关键词
D O I
10.1021/la070089n
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Well-defined glycopolymers containing linear and cyclic carbohydrate moieties as pendent groups were prepared by reversible addition fragmentation chain transfer polymerization (RAFT). The RAFT synthesized glycopolymers were used for the aqueous synthesis of stabilized glyconanoparticles. The in situ reduction of the glycopolymers and HAuCl4 resulted in the formation of highly stable modified gold nanoparticles with diameters ranging from 40 to 80 nm in aqueous media. Multifunctional glyconanoparticles were also generated in the presence of varying amounts of biotinylated-polyethyleneglycol (bio-PEG-SH) having terminal thiol groups. The gold nanoparticles underwent aggregation in the presence of streptavidin as revealed by UV-vis spectroscopy. The availability of the biotin for conjugation to streptavidin was also confirmed using surface plasmon resonance (SPR).
引用
收藏
页码:5056 / 5061
页数:6
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