Characterization of SCAR markers of Eimeria spp. of domestic fowl and construction of a public relational database -: (The Eimeria SCARdb)

被引：29
作者：
Fernandez, S
Katsuyama, AM
Kashiwabara, AY
Madeira, AMBN
Durham, AM
Gruber, A
机构：
[1] USP, Fac Med Vet & Zootecnia, Dept Patol, BR-05508000 Sao Paulo, Brazil
[2] Univ Sao Paulo, Inst Metemat & Estatistica, BR-05508900 Sao Paulo, Brazil
来源：
FEMS MICROBIOLOGY LETTERS | 2004年 / 238卷 / 01期
基金：
巴西圣保罗研究基金会;
关键词：
Eimeria; sequence-characterized amplified region; molecular markers; relational database; domestic fowl; coccidiosis;
D O I：
10.1016/j.femsle.2004.07.034
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
This study reports the development and characterization of 151 sequence characterized amplified region (SCAR) markers for the seven Eimeria species that infect the domestic fowl. From this set, 84 markers are species-specific and 67 present partial specificity. The complete nucleotide sequence was derived for all markers, revealing the presence of micro- and minisatellite repetitive units in 22 SCARs, with up to five distinct repeat units being observed per marker. Only 15 markers showed significant hits in similarity searches against public sequence databases, thus confirming their anonymous and non-coding character. Finally, a relational database of the markers (the Eimeria SCARdb) was developed and made available on the Internet, providing a valuable resource of SCAR markers that can be useful for molecular diagnosis, and also for epizootiological, genetic variability and genome mapping studies. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：183 / 188
页数：6
相关论文
共 30 条
[1]   Recent advances in biology and immunobiology of Eimeria species and in diagnosis and control of infection with these coccidian parasites of poultry [J].
Allen, PC ;
Fetterer, RH .
CLINICAL MICROBIOLOGY REVIEWS, 2002, 15 (01) :58-+
[2]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[3]   Phylogenetic relationships among eight Eimeria species infecting domestic fowl inferred using complete small subunit ribosomal DNA sequences [J].
Barta, JR ;
Martin, DS ;
Liberator, PA ;
Dashkevicz, M ;
Anderson, JW ;
Feighner, SD ;
Elbrecht, A ;
PerkinsBarrow, A ;
Jenkins, MC ;
Danforth, HD ;
Ruff, MD ;
ProfousJuchelka, H .
JOURNAL OF PARASITOLOGY, 1997, 83 (02) :262-271
[4]   Tandem repeats finder: a program to analyze DNA sequences [J].
Benson, G .
NUCLEIC ACIDS RESEARCH, 1999, 27 (02) :573-580
[5]   Identification of six Trypanosoma cruzi lineages by sequence-characterised amplified region markers [J].
Brisse, S ;
Dujardin, JC ;
Tibayrenc, M .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2000, 111 (01) :95-105
[6]   A new approach for the identification and the diagnosis of Eimeria media parasite of the rabbit [J].
Cere, N ;
Humbert, JF ;
Licois, D ;
Corvione, M ;
Afanassieff, M ;
Chanteloup, N .
EXPERIMENTAL PARASITOLOGY, 1996, 82 (02) :132-138
[7]   Base-calling of automated sequencer traces using phred.: II.: Error probabilities [J].
Ewing, B ;
Green, P .
GENOME RESEARCH, 1998, 8 (03) :186-194
[8]   Base-calling of automated sequencer traces using phred.: I.: Accuracy assessment [J].
Ewing, B ;
Hillier, L ;
Wendl, MC ;
Green, P .
GENOME RESEARCH, 1998, 8 (03) :175-185
[9]   A multiplex PCR assay for the simultaneous detection and discrimination of the seven Eimeria species that infect domestic fowl [J].
Fernandez, S ;
Pagotto, AH ;
Furtado, MM ;
Katsuyama, AM ;
Madeira, AMBN ;
Gruber, A .
PARASITOLOGY, 2003, 127 :317-325
[10]   A survey of the inter- and intraspecific RAPD markers of Eimeria spp. of the domestic fowl and the development of reliable diagnostic tools [J].
Fernandez, S ;
Costa, AC ;
Katsuyama, AM ;
Madeira, AMBN ;
Gruber, A .
PARASITOLOGY RESEARCH, 2003, 89 (06) :437-445
123