Effect of regulatory T cells and adherent cells on the expansion of HBcAg-specific CD8+ T cells in patients with chronic hepatitis B virus infection

被引：30
作者：
Li, Xuefen [2 ]
Chen, Yu [1 ]
Ma, Zhaowen [3 ]
Ye, Bo [2 ]
Wu, Wei [1 ]
Li, Lanjuan [1 ]
机构：
[1] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Sta Key Lab Diag & Treatment Infect Dis, Hangzhou 310003, Zhejiang, Peoples R China
[2] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Dept Lab Med, Hangzhou 310003, Zhejiang, Peoples R China
[3] ZaoZhuang Matern & Child Hlth Hosp, Lab Mol Immunopathol, Jinan, Shandong, Peoples R China
来源：
CELLULAR IMMUNOLOGY | 2010年 / 264卷 / 01期
关键词：
Chronic HBV infection; HBc18-27-specific CD8(+) T cells; Regulatory T cell; Adherent cell; DENDRITIC CELLS; PERIPHERAL-BLOOD; IN-VIVO; IMMUNE-RESPONSES; VIRAL-HEPATITIS; LYMPHOCYTES; ACTIVATION; PATHOGENESIS; INDUCTION; COMPLEXES;
D O I：
10.1016/j.cellimm.2010.04.009
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Patients with chronic HBV infection show poor immune response to HBV-specific CD8(+) T cells. Several studies demonstrate that regulatory T cells (Treg) and dendritic cells (DC) are important to maintain peripheral immune tolerance. In this study, we investigated the effects of CD4(+)CD25(+)Treg and/or the adherent cells (AC) on the proliferation of HBc18-27-specific CD8(+) T cells (c18-27-CD8T5) in response to in vitro stimulation. The frequency of c18-27-CD8Ts in four different mixed leukocyte reactions (MLRs) were analyzed using an HLA-A2-HBc18-27 tetramer. The data indicated that the median percentage of c18-27-CD8Ts in four different MLRs were significant difference in patients with chronic HBV infection. Our results showed that Treg and/or AC might suppress the frequency of HBc18-27-specific CD8(+) T cell proliferation in response to in vitro stimulation in chronic HBV patients, and AC might be more effective than Treg. (C) 2010 Elsevier Inc. All rights reserved.
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收藏
页码：42 / 46
页数：5
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