Improvement of artemisinin production by chitosan in hairy root cultures of Artemisia annua L.

被引:106
|
作者
Putalun, Waraporn [1 ]
Luealon, Wanwimon
De-Eknamkul, Wanchai
Tanaka, Hiroyuki
Shoyama, Yukihiro
机构
[1] Khon Kaen Univ, Fac Pharmaceut Sci, Khon Kaen 40002, Thailand
[2] Chulalongkorn Univ, Fac Pharmaceut Sci, Bangkok 10330, Thailand
[3] Kyushu Univ, Grad Sch Pharmaceut Sci, Higashi Ku, Fukuoka 8128582, Japan
基金
日本学术振兴会;
关键词
Artemisia annua L; artemisinin; elicitors; hairy roots;
D O I
10.1007/s10529-007-9368-8
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Artemisinin production by hairy roots of Artemisia annua L. was increased 6-fold to 1.8 mu g mg(-1) dry wt over 6 days by adding 150 mg chitosan l(-1). The increase was dose-dependent. Similar treatment of hairy roots with methyl jasmonate (0.2 mM) or yeast extract (2 mg ml(-1)) increased artemisinin production to 1.5 and 0.9 mu g mg(-1) dry wt, respectively.
引用
收藏
页码:1143 / 1146
页数:4
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