Improvement of artemisinin production by chitosan in hairy root cultures of Artemisia annua L.

被引：106

作者：

Putalun, Waraporn ^{[1
]}

Luealon, Wanwimon

De-Eknamkul, Wanchai

Tanaka, Hiroyuki

Shoyama, Yukihiro

机构：

[1] Khon Kaen Univ, Fac Pharmaceut Sci, Khon Kaen 40002, Thailand

[2] Chulalongkorn Univ, Fac Pharmaceut Sci, Bangkok 10330, Thailand

[3] Kyushu Univ, Grad Sch Pharmaceut Sci, Higashi Ku, Fukuoka 8128582, Japan

来源：

BIOTECHNOLOGY LETTERS | 2007年 / 29卷 / 07期

基金：

日本学术振兴会;

关键词：

Artemisia annua L; artemisinin; elicitors; hairy roots;

D O I：

10.1007/s10529-007-9368-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Artemisinin production by hairy roots of Artemisia annua L. was increased 6-fold to 1.8 mu g mg(-1) dry wt over 6 days by adding 150 mg chitosan l(-1). The increase was dose-dependent. Similar treatment of hairy roots with methyl jasmonate (0.2 mM) or yeast extract (2 mg ml(-1)) increased artemisinin production to 1.5 and 0.9 mu g mg(-1) dry wt, respectively.

引用

页码：1143 / 1146

页数：4

共 9 条

[1] Methyl jasmonate and salicylic acid elicitation induces ginsenosides accumulation, enzymatic and non-enzymatic antioxidant in suspension culture Panax ginseng roots in bioreactors
Ali, Mohammad Babar
Yu, Kee-Won
Hahn, Eun-Joo
Paek, Kee-Yoeup
[J]. PLANT CELL REPORTS, 2006, 25 (06) : 613 - 620
[2] Improvement of phenylethanoid glycosides biosynthesis in Cistanche deserticola cell suspension cultures by chitosan elicitor
Cheng, XY
Zhou, HY
Cui, X
Ni, W
Liu, CZ
[J]. JOURNAL OF BIOTECHNOLOGY, 2006, 121 (02) : 253 - 260
[3] QINGHAOSU (ARTEMISININ) - AN ANTIMALARIAL DRUG FROM CHINA
KLAYMAN, DL
[J]. SCIENCE, 1985, 228 (4703) : 1049 - 1055
[4] Improvement of artemisinin accumulation in hairy root cultures of Artemisia annua L by fungal elicitor
Liu, C
Wang, Y
Xu, X
Ouyang, F
Ye, H
Li, G
[J]. BIOPROCESS ENGINEERING, 1999, 20 (02): : 161 - 164
[5] Artemisinin production by Artemisia annua L-transformed organ cultures
Paniego, NB
Giulietti, AM
[J]. ENZYME AND MICROBIAL TECHNOLOGY, 1996, 18 (07) : 526 - 530
[6] PUTALUN W, 2006, 1 SIN THAI C TRAD ME
[7] Improved artemisinin accumulation in hairy root cultures of Artemisia annua by (22S, 23S)-homobrassinolide
Wang, JW
Kong, FX
Tan, RX
[J]. BIOTECHNOLOGY LETTERS, 2002, 24 (19) : 1573 - 1577
[8] WEATHERS PJ, 1994, BIOTECHNOL LETT, V16, P1281
[9] Elicitor signal transduction leading to production of plant secondary metabolites
Zhao, J
Davis, LC
Verpoorte, R
[J]. BIOTECHNOLOGY ADVANCES, 2005, 23 (04) : 283 - 333

← 1 →