Study of molecular genetic diversity and evolutionary history of medicinally important endangered genus Chlorophytum using DNA barcodes

被引：6

作者：

Patil, Swapnil M. ^{[1
]}

Rane, Niraj R. ^{[1
]}

Adsul, Avinash A. ^{[2
]}

Gholave, Avinash R. ^{[2
]}

Yadav, Shrirang R. ^{[2
]}

Jadhav, Jyoti P. ^{[1
]}

Govindwar, Sanjay P. ^{[3
]}

机构：

[1] Shivaji Univ, Dept Biotechnol, Kolhapur 416004, Maharashtra, India

[2] Shivaji Univ, Dept Bot, Kolhapur 416004, Maharashtra, India

[3] Shivaji Univ, Dept Biochem, Kolhapur 416004, Maharashtra, India

来源：

BIOCHEMICAL SYSTEMATICS AND ECOLOGY | 2016年 / 65卷

关键词：

Plant DNA barcoding; Marker screening; DGGE; Pseudogenes; SNP; Chlorophytum; MULTIPLE SEQUENCE ALIGNMENT; NUCLEOTIDE; PATTERNS; PLANTS;

D O I：

10.1016/j.bse.2016.02.035

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study was aimed to authenticate and present phylogenetic relationship among 19 species of genus Chlorophytum using DNA barcoding. In all, 107 accessions were analyzed with eight plastid (matK, rbcL, trnH-psbA, rpoC1, ycf5, rpoB, atp and psbK-psbI) and six nuclear (ITS) markers. The matK and rbcL were found to be ideal markers for identification and discrimination of Chlorophytum species. Phylogenetic analysis based on matK and rbcL sequences resolved the species in two major clades. All markers, except matK and rbcL, showed ambiguous reads and paralogy in analysis. DGGE analysis showed the presence of pseudogenes and/or co-amplification in these markers, which caused poor sequence quality. Phylogeny and probable evolution of genus Chlorophytum was proposed on the basis of cytological, morphological and genetic information. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：245 / 252

页数：8

共 8 条

[1] A Systematic Study on DNA Barcoding of Medicinally Important Genus Epimedium L. (Berberidaceae)
Guo, Mengyue
Xu, Yanqin
Ren, Li
He, Shunzhi
Pang, Xiaohui
GENES, 2018, 9 (12)
[2] Molecular identification of the economically important Solanum subgenus Leptostemonum (Solanaceae) using DNA barcodes
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Giraldo, Carlos E.
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JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY, 2022, 31 (4) : 938 - 952
[3] Exploring Genetic Divergence in a Species-Rich Insect Genus Using 2790 DNA Barcodes
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Stur, Elisabeth
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PLOS ONE, 2015, 10 (09):
[4] Genetic Diversity and Demographic History in the Cactophilic Drosophila repleta Species Group (Diptera: Drosophilidae) in North America Inferred from Mitochondrial DNA Barcodes
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JOURNAL OF HEREDITY, 2019, 110 (01) : 34 - 45
[5] Assessment of Genetic Diversity and Evaluation of Relatedness Through Morphological and Molecular Markers Among Medicinally Important Trees: Terminalia arjuna, T. bellerica, T. catappa and T. chebula
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NATIONAL ACADEMY SCIENCE LETTERS-INDIA, 2019, 42 (02): : 155 - 159
[6] Analysis of Genetic Diversity and Phylogenetic Relationships of Wheat (Triticum aestivum L.) Genotypes Using Phenological, Molecular and DNA Barcoding Markers
El-Esawi, Mohamed A.
Elashtokhy, Mohamed M. A.
Shamseldin, Sahar A. M.
El-Ballat, Enas M.
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Heikal, Yasmin M.
GENES, 2023, 14 (01)
[7] Revealing hidden species diversity in closely related species using nuclear SNPs, SSRs and DNA sequences – a case study in the tree genus Milicia
Kasso Daïnou
Céline Blanc-Jolivet
Bernd Degen
Priscilla Kimani
Dyana Ndiade-Bourobou
Armel S. L. Donkpegan
Félicien Tosso
Esra Kaymak
Nils Bourland
Jean-Louis Doucet
Olivier J. Hardy
BMC Evolutionary Biology, 16
[8] Revealing hidden species diversity in closely related species using nuclear SNPs, SSRs and DNA sequences - a case study in the tree genus Milicia
Dainou, Kasso
Blanc-Jolivet, Celine
Degen, Bernd
Kimani, Priscilla
Ndiade-Bourobou, Dyana
Donkpegan, Armel S. L.
Tosso, Felicien
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Bourland, Nils
Doucet, Jean-Louis
Hardy, Olivier J.
BMC EVOLUTIONARY BIOLOGY, 2016, 16 : 1 - 15

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