Custom-made novel biomimetic composite scaffolds for the bone regenerative medicine

被引：6

作者：

Ciocca, L. ^{[1
]}

Donati, D. ^{[2
]}

Lesci, I. G. ^{[3
]}

Dozza, B. ^{[2
]}

Duchi, S. ^{[2
]}

Mezini, O. ^{[3
]}

Spadari, A. ^{[4
]}

Romagnoli, N. ^{[4
]}

Scotti, R. ^{[1
]}

Roveri, N. ^{[3
]}

机构：

[1] Univ Bologna, Alma Mater Studiorum, Dept Biomed & Neuromotor Sci, Bologna, Italy

[2] Univ Bologna, Alma Mater Studiorum, Dept Biomed & Neuromotor Sci, Osteoarticular Regenerat Lab,Rizzoli Orthopaed In, Bologna, Italy

[3] Univ Bologna, Dept Chem G Ciamician, Bologna, Italy

[4] Univ Bologna, Alma Mater Studiorum, Fac Vet Med, Bologna, Italy

来源：

MATERIALS LETTERS | 2014年 / 136卷

关键词：

Biomaterial engineering; Composite scaffold; Rapid prototyping; Cell seeding;

D O I：

10.1016/j.matlet.2014.08.097

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

The aim of this pilot study was to test a new customized biomimetic composite scaffolds to restore bone defects using CAD-CAM technology. The new biomaterial was synthesized using biomimetic mineralized collagen, and poly (epsilon-caprolactone) (PCL). The mold used in scaffolding, the surgical guide, and the bony plate used during the surgery, were rapid prototyped (CAD-CAM technology). Our in vitro results showed that sheep mesenchymal stem cells (MSC) seeding efficiency was very high (>94%). Furthermore, cells can adhere, grow and maintain cell viability up to 7 days of in vitro cultivation on the scaffold as revealed by SEM and confocal laser scanning microscope analysis. These results are indicative of good scaffold biocompatibility. The use of the sheep experimental model allowed exploring the efficacy of the customized bony plate used to give extra mechanical support to the scaffold, the potential of CAD-CAM technology in customization of the scaffold, and the in vivo biomimetic properties of the composite biomaterial. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：393 / 396

页数：4

共 6 条

[1] Mesenchymal Stem Cells and Platelet Gel Improve Bone Deposition within CAD-CAM Custom-Made Ceramic HA Scaffolds for Condyle Substitution
Ciocca, L.
Donati, D.
Ragazzini, S.
Dozza, B.
Rossi, F.
Fantini, M.
Spadari, A.
Romagnoli, N.
Landi, E.
Tampieri, A.
Piattelli, A.
Iezzi, G.
Scotti, R.
[J]. BIOMED RESEARCH INTERNATIONAL, 2013, 2013
[2] Gabelnici H.L., 1983, ADV HUMAN FERTILITY, P149
[3] New evidence of surface mineralization of collagen fibrils in wild type zebrafish skeleton by AFM and TEM
Ge, Jun
Cui, Fu-Zhai
Wang, Xiumei
Wang, Yu
[J]. MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS, 2007, 27 (01): : 46 - 50
[4] TITANIUM MINIPLATE FIXATION IN FREE FLAP MANDIBLE RECONSTRUCTION
HIDALGO, DA
[J]. ANNALS OF PLASTIC SURGERY, 1989, 23 (06) : 498 - 507
[5] The Posterior Iliac Crest Outperforms the Anterior Iliac Crest When Obtaining Mesenchymal Stem Cells from Bone Marrow
Pierini, Michela
Di Bella, Claudia
Dozza, Barbata
Frisoni, Tommaso
Martella, Elisa
Bellotti, Chiara
Remondini, Daniel
Lucarelli, Enrico
Giannini, Sandro
Donati, Davide
[J]. JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 2013, 95A (12) : 1101 - 1107
[6] Preparation of a hemiporous hydroxyapatite scaffold and evaluation as a cell-mediated bone substitute
Yoo, Jeong Joon
Kim, Hee Joong
Seo, Sang-Min
Oh, Kyung-Sik
[J]. CERAMICS INTERNATIONAL, 2014, 40 (02) : 3079 - 3087

← 1 →