Beneficial effects of fermented vegetal beverages on human gastrointestinal microbial ecosystem in a simulator

被引：44

作者：

Bianchi, Fernanda ^{[1
]}

Rossi, Elizeu Antonio ^{[1
]}

Sakamoto, Isabel Kimiko ^{[2
]}

Tallarico Adorno, Maria Angela ^{[2
]}

Van de Wiele, Tom ^{[3
]}

Sivieri, Katia ^{[1
]}

机构：

[1] UNESP, Fac Pharmaceut Sci, Probiot Res Lab, Dept Food & Nutr, BR-14801902 Araraquara, SP, Brazil

[2] Sch Engn Sao Carlos EESC USP, Lab Biol Proc LPB, Sao Carlos, SP, Brazil

[3] Univ Ghent, Lab Microbial Ecol & Technol LabMET, B-9000 Ghent, Belgium

来源：

FOOD RESEARCH INTERNATIONAL | 2014年 / 64卷

基金：

巴西圣保罗研究基金会;

关键词：

SHIME (R); Fermented beverage; Lactobacillus casei Lc-01; PCR-DGGE; SCFA; CHAIN FATTY-ACIDS; FECAL MICROBIOTA; GUT MICROBIOTA; BACTERIA; ENUMERATION; METABOLISM; DIVERSITY; INTESTINE; COMMUNITY; SELECTION;

D O I：

10.1016/j.foodres.2014.05.072

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The aim of this study was to evaluate the effects of four beverage formulations (prebiotic - fructooligosaccharide, probiotic - Lactobacillus casei Lc-01, synbiotic - fructooligosaccharide and L. casei Lc-01 and placebo) based on aqueous extracts of soy and quinoa, towards the human intestinal microbiota using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME (R)), a dynamic model of the human gut. To monitor the effects on microbial community composition, plate counts on specific growth media and a PCR-DGGE analysis were performed on samples from all colon compartments - ascending, transverse and descending. To verify the effects on microbial metabolism, we analyzed the ammonium and short chain fatty acids (SCFAs) concentrations. The synbiotic beverage showed the best microbiological results in the ascending colon compartment, stimulating the growth of Lactobacillus spp. and Bifidobacterium spp., and reducing Clostridium spp., Bacteroides spp., enterobacteria and Enterococcus spp. populations in this compartment. A larger reduction (p < 0.05) of ammonia ions in the ascending colon was observed during the synbiotic beverage treatment. No statistical difference was observed in SCFA production among the treatments and the basal period. Plate count and DGGE analysis showed the survival of L. casei Lc-01 in the colon. DGGE analysis also showed higher richness and diversity of the Lactobacillus spp. community during the treatment with synbiotic beverage, with higher accentuation in the ascending colon. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：43 / 52

页数：10

共 54 条

[1]

ALLISON C, 1988, J GEN MICROBIOL, V134, P1397

[2] Food components in fractions of quinoa seed [J].

Ando, H ;

Chen, YC ;

Tang, HJ ;

Shimizu, M ;

Watanabe, K ;

Mitsunaga, T .

FOOD SCIENCE AND TECHNOLOGY RESEARCH, 2002, 8 (01) :80-84

[3]

Bedani R, 2008, THESIS UNESP SAO PAU, P140

[4] Effects of probiotic administration upon the composition and enzymatic activity of human fecal microbiota in patients with irritable bowel syndrome or functional diarrhea [J].

Brigidi, P ;

Vitali, B ;

Swennen, E ;

Bazzocchi, G ;

Matteuzzi, D .

RESEARCH IN MICROBIOLOGY, 2001, 152 (08) :735-741

[5] Effect of cryoprotectants, prebiotics and microencapsulation on survival of probiotic organisms in yoghurt and freeze-dried yoghurt [J].

Capela, P ;

Hay, TKC ;

Shah, NP .

FOOD RESEARCH INTERNATIONAL, 2006, 39 (02) :203-211

[6] Correlations between molecular and operational parameters in continuous lab-scale anaerobic reactors [J].

Carballa, Marta ;

Smits, Marianne ;

Etchebehere, Claudia ;

Boon, Nico ;

Verstraete, Willy .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2011, 89 (02) :303-314

[7] Effects of encapsulated Lactobacillus acidophilus along with pasteurized longan juice on the colon microbiota residing in a dynamic simulator of the human intestinal microbial ecosystem [J].

Chaikham, Pittaya ;

Apichartsrangkoon, Arunee .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2014, 98 (01) :485-495

[8] Intestinal luminal nitrogen metabolism: Role of the gut microbiota and consequences for the host [J].

Davila, Anne-Marie ;

Blachier, Francois ;

Gotteland, Martin ;

Andriamihaja, Mireille ;

Benetti, Pierre-Henri ;

Sanz, Yolanda ;

Tome, Daniel .

PHARMACOLOGICAL RESEARCH, 2013, 68 (01) :95-107

[9]

De Boever P, 2000, J NUTR, V130, P2599

[10]

Desai AR, 2004, J FOOD SCI, V69, pM57, DOI 10.1111/j.1365-2621.2004.tb13371.x

← 1 2 3 4 5 6 →