Islet transplantation restores the damage of glomerulus filtration membrane in a rat model of streptozotocin-induced diabetic nephropathy

被引：0
作者：
Xu Ziqiang [1 ]
He Yunqiang [1 ]
Fu Hongxing [1 ]
Wang Jinjun [1 ]
Cai Yong [1 ]
机构：
[1] Wenzhou Med Univ, Affiliated Hosp 1, Transplantat Ctr, Wenzhou, Peoples R China
来源：
JOURNAL OF THE PAKISTAN MEDICAL ASSOCIATION | 2016年 / 66卷 / 03期
关键词：
Diabetic nephropathy; Islet transplantation; Podocytes; Glomerular basement membrane; TGF-beta; 1; PATHOGENESIS; PROTEINURIA; PODOCYTES; DISEASE;
D O I：
暂无
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Objective: To evaluate the effects on filtration membrane of glomerulus after islet transplantation in a rat model of streptozotocin-induced diabetic nephropathy. Methods: The experimental case-control study was conducted at Wenzhou Medical University, Wenzhou, China from January to May 2015, and comprised male Sprague Dawley rats obtained from the Laboratory Animal Centre of Wenzhou Medical University. The rats were intraperitoneally injected with streptozotocin to induce diabetic nephropathy. Diabetic rats were divided into two groups; the islets group received islets transplantation under the kidney capsule; and the diabetic nephropathy (DN) group consisted of untreated diabetic nephropathy rats. The control group consisted of non-diabetic rats. Islets were surgically transplanted under the kidney capsule. Kidney function and blood glucose were measured and pathological changes in the kidney were observed by electron microscope, while the expressions of Wilms' tumour-1, caspase-3 and transforming growth factor-beta 1 were tested by immunohistochemical method and Western blot analysis. Results: Each of the three groups had 6 rats each with body weights ranging from 180g to 220g. Reduced urinary protein excretion and alleviated damage of podocytes and glomerular basement membrane were seen in the islet transplanted rats. The alleviation of podocyte damage was related to alteration in the synthesis of caspase-3, transforming growth factor-beta 1, and Wilms' tumour-1 protein in the glomerulus. Conclusion: Diabetic nephropathy rats after islet transplantation can ameliorate the damage of podocytes and basement membrane by inhibiting the pathway of transforming growth factor-beta 1.
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页码：296 / 301
页数：6
相关论文
共 22 条
[1]  
[Anonymous], 2009, J NEPHROL SOC THAI
[2]   Temporal Trends in the Prevalence of Diabetic Kidney Disease in the United States [J].
de Boer, Ian H. ;
Rue, Tessa C. ;
Hall, Yoshio N. ;
Heagerty, Patrick J. ;
Weiss, Noel S. ;
Himmelfarb, Jonathan .
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2011, 305 (24) :2532-2539
[3]   Islet Transplantation and Insulin Administration Relieve Long-Term Complications and Rescue the Residual Endogenous Pancreatic β Cells [J].
Figliuzzi, Marina ;
Bianchi, Roberto ;
Cavagnini, Cristina ;
Lombardi, Raffaella ;
Porretta-Serapiglia, Carla ;
Lauria, Giuseppe ;
Avezza, Federica ;
Canta, Annalisa ;
Carozzi, Valentina ;
Chiorazzi, Alessia ;
Marmiroli, Paola ;
Meregalli, Cristina ;
Oggioni, Norberto ;
Sala, Barbara ;
Cavaletti, Guido ;
Remuzzi, Andrea .
AMERICAN JOURNAL OF PATHOLOGY, 2013, 183 (05) :1527-1538
[4]   Long-term gene expression and metabolic control exerted by lentivirus-transduced pancreatic islets [J].
He, Zhong ;
Wang, Feng ;
Kumagai-Braesch, Makiko ;
Permert, Johan ;
Holgersson, Jan .
XENOTRANSPLANTATION, 2006, 13 (03) :195-203
[5]   Proteinuria in diabetic kidney disease: A mechanistic viewpoint [J].
Jefferson, J. A. ;
Shankland, S. J. ;
Pichler, R. H. .
KIDNEY INTERNATIONAL, 2008, 74 (01) :22-36
[6]  
Jiao B, 2013, BIOMED AGING PATHOL, V3, P36
[7]   Combined effects of moderately elevated blood glucose and locally produced TGF-β1 on glomerular morphology and renal collagen production [J].
Krag, Soren ;
Nyengaard, Jens R. ;
Wogensen, Lise .
NEPHROLOGY DIALYSIS TRANSPLANTATION, 2007, 22 (09) :2485-2496
[8]   Endothelial cell and podocyte autophagy synergistically protect from diabetes-induced glomerulosclerosis [J].
Lenoir, Olivia ;
Jasiek, Magali ;
Henique, Carole ;
Guyonnet, Lea ;
Hartleben, Bjoern ;
Bork, Tillmann ;
Chipont, Anna ;
Flosseau, Kathleen ;
Bensaada, Imane ;
Schmitt, Alain ;
Masse, Jean-Marc ;
Souyri, Michele ;
Huber, Tobias B. ;
Tharaux, Pierre-Louis .
AUTOPHAGY, 2015, 11 (07) :1130-1145
[9]   Organogenesis of the kidney glomerulus Focus on the glomerular basement membrane [J].
Miner, Jeffrey H. ;
Towler, Dwight ;
Chen, Feng ;
Kopan, Raphael ;
Hruska, Keith .
ORGANOGENESIS, 2011, 7 (02) :75-82
[10]   The MCP-1/CCR2 axis in podocytes is involved in apoptosis induced by diabetic conditions [J].
Nam, Bo Young ;
Paeng, Jisun ;
Kim, Seung Hye ;
Lee, Sun Ha ;
Kim, Do Hee ;
Kang, Hye-Young ;
Li, Jin Ji ;
Kwak, Seung-Jae ;
Park, Jung Tak ;
Yoo, Tae-Hyun ;
Han, Seung Hyeok ;
Kim, Dong Ki ;
Kang, Shin-Wook .
APOPTOSIS, 2012, 17 (01) :1-13
123