Sample stacking for the analysis of catechins by microemulsion EKC

被引:17
作者
Huang, Hsi-Ya [1 ]
Huang, I.-Yun
Liang, Hsin-hui
Lee, Szetsen
机构
[1] Chung Yuan Christian Univ, Dept Chem, Chungli, Taiwan
[2] Chung Yuan Christian Univ, R&D Ctr Membrane Technol, Chungli, Taiwan
[3] Chung Yuan Christian Univ, Ctr Nanotechnol, Dept Chem, Chungli, Taiwan
关键词
catechins; microemulsion EKC; on-line concentration; sample stacking;
D O I
10.1002/elps.200600413
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In this study, an on-line concentration method, ASEI (anion-selective exhaustive injection)sweeping technology which was coupled with microemulsion EKC (MEEKC), was used to analyze and detect six catechins ((-)-epicatechin, (+)-catechin, (-)-epigallocatechin gallate, (-)-epicatechin gallate, (-)-epigallocatechin, and (-)-gallocatechin). In addition to the effects of the buffer pH and electrolyte concentration on stacking, the compositions of microemulsion (types of oil phase, and types and levels of cosurfactant) also dominated the stacking effect of catechins. In MEEKC, the effect of the type of oil in microemulsion on separation mechanism is often unclear. This study had demonstrated that the oil type in microemulsion indeed altered the affinity of oil droplets with analytes. Finally, this proposed ASEI-sweeping MEEKC method was able to detect trace level of catechins in food products that was not previously possible by a normal MEEKC method.
引用
收藏
页码:1735 / 1743
页数:9
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