Real-Time Monitoring the Cytotoxic Effect of Andrographolide on Human Oral Epidermoid Carcinoma Cells

被引：8

作者：

Liao, Heng-Yi ^{[1
]}

Huang, Chun-Chung ^{[2
,3
]}

Chao, Shih-Chi ^{[3
,4
]}

Chiang, Chien-Ping ^{[5
,6
]}

Tang, Bo-Hsuan ^{[7
]}

Lee, Shiao-Pieng ^{[1
,8
]}

Wang, Jehng-Kang ^{[1
,5
]}

机构：

[1] Natl Def Med Ctr, Grad Inst Life Sci, Taipei 114201, Taiwan

[2] Tamkang Univ, Dept Chem, New Taipei 112304, Taiwan

[3] Chung Shan Med Univ, Inst Oral Sci, Taichung 40201, Taiwan

[4] Lotung Poh Ai Hosp, Lo Hsu Med Fdn, Dept Med Res & Educ, Yilan 265501, Taiwan

[5] Natl Def Med Ctr, Dept Biochem, Taipei 114201, Taiwan

[6] Triserv Gen Hosp, Dept Dermatol, Taipei 114202, Taiwan

[7] Shih Chien Univ, Informat Technol & Management, Taipei 104336, Taiwan

[8] Triserv Gen Hosp, Dept Dent, Div Oral & Maxillofacial Surg, Taipei 114202, Taiwan

来源：

BIOSENSORS-BASEL | 2022年 / 12卷 / 05期

关键词：

oral squamous carcinoma; andrographolide; electric cell-substrate impedance sensing; FACTOR-KAPPA-B; BCL-2; FAMILY; C-ELEGANS; CANCER; APOPTOSIS; SUPPRESSION; ACTIVATION; DNA; INFLAMMATION; INHIBITION;

D O I：

10.3390/bios12050304

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Andrographolide is an active diterpenoid compound extracted from Andrographis paniculata. It exhibits antiinflammatory and anticancer effects. Previous studies show that it is non-toxic to experimental animals. The leading causes of cancer are chronic inflammation and high blood glucose. This study determines the cytotoxic effect of andrographolide on cellular morphology, viability, and migration for human oral epidermoid carcinoma cell Meng-1 (OEC-M1). We use electric cellsubstrate impedance sensing (ECIS) to measure the subsequent overall impedance changes of the cell monolayer in response to different concentrations of andrographolide for 24 h (10-100 mu M). The results for exposure of OEC-M1 cells to andrographolide (10-100 mu M) for 24 h show a concentration-dependent decrease in the overall measured resistance at 4 kHz. AlamarBlue cell viability assay and annexin V also show the apoptotic effect of andrographolide on OEC-M1 cells. A reduction in wound-healing recovery rate is observed for cells treated with 30 mu M andrographolide. This study demonstrates that ECIS can be used for the in vitro screening of anticancer drugs. ECIS detects the cytotoxic effect of drugs earlier than traditional biochemical assays, and it is more sensitive and shows more detail.

引用

页数：12

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