Surface-specific washing-free immunosensor for time-resolved cortisol monitoring

被引：10

作者：

Safarian, Sofia M. ^{[1
]}

Kusov, Pavel A. ^{[1
]}

Kosolobov, Sergey S. ^{[1
]}

Borzenkova, Oksana, V ^{[1
]}

Khakimov, Artem, V ^{[1
]}

Kotelevtsev, Yuri, V ^{[1
]}

Drachev, Vladimir P. ^{[1
,2
]}

机构：

[1] Skolkovo Inst Sci & Technol, 3 Nobel St, Moscow 143025, Russia

[2] Univ North Texas, Denton, TX 76203 USA

来源：

TALANTA | 2021年 / 225卷

关键词：

Competitive immunoassay; Metal enhanced fluorescence; Cortisol; Real-time measurement; Gold nanostructures; BSA-cortisol conjugate; IMMUNOASSAY; FLUORESCENCE; BRAIN; ASSAY;

D O I：

10.1016/j.talanta.2020.122070

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Cortisol is a steroid hormone that regulates a wide range of vital processes. Its level changes with diurnal rhythm and reacts to stress. Measurement of cortisol levels is still a complex multi-step process. A reversible washing-free registration method is required. Here we describe metal-enhanced fluorescence assay based on a displacement of a dye labeled BSA-cortisol conjugate from the immune complex immobilized on the golden islands by free cortisol. This competitive approach allows time-resolved monitoring of the fluorescent signal, surface-enhanced by the gold film, and provides the possibility of continuous real-time cortisol monitoring based on the implantable surface-enhanced immunosensor, which was not demonstrated so far even in vitro.

引用

页数：7