How to Obtain a High Quality ctDNA in Lymphoma Patients: Preanalytical Tips and Tricks

被引：8

作者：

Bourbon, Estelle ^{[1
]}

Alcazer, Vincent ^{[1
]}

Cheli, Estelle ^{[1
]}

Huet, Sarah ^{[1
,2
]}

Sujobert, Pierre ^{[1
,2
]}

机构：

[1] Hosp Civils Lyon, Serv Hematol Biol, Hop Lyon Sud, F-69495 Lyon, France

[2] Univ Lyon 1, Lymphoma Immunobiol Team, Fac Med & Maieut Lyon Sud Charles Merieux, F-69921 Oullins, France

来源：

PHARMACEUTICALS | 2021年 / 14卷 / 07期

关键词：

ctDNA; lymphoma; preanalytical; CELL-FREE-DNA; CIRCULATING TUMOR DNA; TUBE SYSTEM TRANSPORT; PLASMA DNA; CANCER; URINE; EXTRACTION; MUTATIONS; STORAGE; ORIGIN;

D O I：

10.3390/ph14070617

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The analysis of circulating tumor DNA (ctDNA) released by tumor cells holds great promise for patients with lymphoma, to refine the diagnostic procedure, clarify the prognosis, monitor the response to treatment, and detect relapses earlier. One of the main challenges of the coming years is to adapt techniques from highly specialized translational teams to routine laboratories as this requires a careful technical and clinical validation, and we have to achieve this as fast as possible to transform a promising biomarker into a routine analysis to have a direct consequence on patient care. Whatever the analytical technology used, the prerequisite is to obtain high yields of ctDNA of optimal quality. In this review, we propose a step-by-step description of the preanalytical process to obtain high-quality ctDNA, emphasizing the technical choices that need to be made and the experimental data that can support these choices.

引用

页数：10

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