Similarities and differences in the influence of polycations and oligomers on DNA conformation and packaging

被引：5

作者：

Kasyanenko, Nina ^{[1
]}

Dribinsky, Boris ^{[1
]}

机构：

[1] St Petersburg State Univ, Dept Phys, 7-9 Univ Skaya Nab, St Petersburg 199034, Russia

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2016年 / 86卷

关键词：

DNA condensation; Poly-L-lysine and polyamines; Phase diagrams; POLY-L-LYSINE; COMPACT FORM; CONDENSATION; COMPLEXES; IONS; COLLAPSE; TRANSITION; POLYLYSINE; MOLECULES; BINDING;

D O I：

10.1016/j.ijbiomac.2016.01.044

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A comparison of DNA conformational changes in a solution containing the poly-L-lysine with the number of monomers z = 3, 5,17, 20, 270,325 and polyamines (spermine and spermidine) was carried out in 1 M and 5 mM NaCl solutions. It was shown that despite the identical results of DNA condensation induced by compounds, their influence on the DNA conformation prior to packaging depends on whether they belong to a long polycations or short oligomers. DNA secondary and tertiary structures were examined using Circular Dichroism, UV-vis Spectrophotometry, Dynamic Light Scattering, Low Gradient Viscometry, Flow Birefringence, and AFM. The phase diagrams for systems of DNA-polycations, DNA-oligomers, DNA-polyamines were drawn. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：216 / 223

页数：8

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