Effects of activin A on the pluripotency of induced pluripotent stem cells derived from porcine Sertoli cells

被引:0
作者
Setthawong, Piyathip [1 ]
Tharasanit, Theerawat [1 ]
Techakumphu, Mongkol [1 ]
机构
[1] Chulalongkorn Univ, Fac Vet Sci, Dept Obstet Gynaecol & Reprod, Bangkok 10330, Thailand
来源
THAI JOURNAL OF VETERINARY MEDICINE | 2019年 / 49卷 / 02期
基金
欧盟地平线“2020”;
关键词
activin A; induced pluripotent stem cells; pluripotency; porcine; Sertoli cells; TGF-beta; 1; inhibitor; SELF-RENEWAL; IPS CELLS; GENERATION; DIFFERENTIATION; EXPRESSION; DERIVATION; PATTERNS; NANOG; LINES; SOX2;
D O I
暂无
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The Activin/Nodal signaling pathway is necessary for promoting the self-renewal and pluripotency in several species. However, these effects in porcine stem cells have yet to be examined. We hypothesized that Activin/Nodal signaling could be an important factor for maintenance of porcine induced pluripotent stem cells (iPSCs). The effects of activin A on pluripotency of porcine iPSCs derived from Sertoli cells were examined. Two iPS cell lines were cultured with different culture conditions (control, activin A, TGF-beta 1 inhibitor and activin A combined with TGF-beta 1 inhibitor). The Sertoli iPSCs treated with different conditions were compared for morphology, alkaline phosphatase (AP) activity, OCT4 protein expression and quantitative expression of genes (endogenous pluripotency genes and cell differentiated genes). This study revealed that the activin A treated iPSCs increased intensity of OCT4 protein expression. Furthermore, expressions of the pluripotent genes including OCT4 and NANOG were significantly upregulated in the activin A treated group (P < 0.05). In contrast, the selective TGF-beta 1 inhibitor adversely affected to iPSCs morphology and patterns of the AP and OCT4 protein expression. The TGF-beta 1 inhibitor also resulted in significant upregulation and downregulation for DES (mesoderm differentiation) and GATA 6 (endoderm differentiation), respectively (P < 0.05) when compared with other groups. In conclusion, activin A significantly upregulated the endogenous expression of OCT4 and NANOG pluripotent genes, thereby improving networks of the pluripotency in porcine iPSCs.
引用
收藏
页码:183 / 191
页数:9
相关论文
共 51 条
[1]   Pig Epiblast Stem Cells Depend on Activin/Nodal Signaling for Pluripotency and Self-Renewal [J].
Alberio, Ramiro ;
Croxall, Nicola ;
Allegrucci, Cinzia .
STEM CELLS AND DEVELOPMENT, 2010, 19 (10) :1627-1636
[2]   Core transcriptional regulatory circuitry in human embryonic stem cells [J].
Boyer, LA ;
Lee, TI ;
Cole, MF ;
Johnstone, SE ;
Levine, SS ;
Zucker, JR ;
Guenther, MG ;
Kumar, RM ;
Murray, HL ;
Jenner, RG ;
Gifford, DK ;
Melton, DA ;
Jaenisch, R ;
Young, RA .
CELL, 2005, 122 (06) :947-956
[3]   Nodal signalling in the epiblast patterns the early mouse embryo [J].
Brennan, J ;
Lu, CC ;
Norris, DP ;
Rodriguez, TA ;
Beddington, RSP ;
Robertson, EJ .
NATURE, 2001, 411 (6840) :965-969
[4]   Pluripotency Network in Porcine Embryos and Derived Cell Lines [J].
Brevini, T. A. L. ;
Pennarossa, G. ;
Maffei, S. ;
Gandolfi, F. .
REPRODUCTION IN DOMESTIC ANIMALS, 2012, 47 :86-91
[5]   Porcine Induced Pluripotent Stem Cells Require LIF and Maintain Their Developmental Potential in Early Stage of Embryos [J].
Cheng, De ;
Guo, Yanjie ;
Li, Zhenzhen ;
Liu, Yajun ;
Gao, Xing ;
Gao, Yi ;
Cheng, Xiang ;
Hu, Junhe ;
Wang, Huayan .
PLOS ONE, 2012, 7 (12)
[6]   Strategies for Improving Animal Models for Regenerative Medicine [J].
Cibelli, Jose ;
Emborg, Marina E. ;
Prockop, Darwin J. ;
Roberts, Michael ;
Schatten, Gerald ;
Rao, Mahendra ;
Harding, John ;
Mirochnitchenko, Oleg .
CELL STEM CELL, 2013, 12 (03) :271-274
[7]   Sox2 signaling in prosensory domain specification and subsequent hair cell differentiation in the developing cochlea [J].
Dabdoub, Alain ;
Puligilla, Chandrakala ;
Jones, Jennifer M. ;
Fritzsch, Bernd ;
Cheah, Kathryn S. E. ;
Pevny, Larysa H. ;
Kelley, Matthew W. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (47) :18396-18401
[8]   Barriers for Deriving Transgene-Free Pig iPS Cells with Episomal Vectors [J].
Du, Xuguang ;
Feng, Tao ;
Yu, Dawei ;
Wu, Yuanyuan ;
Zou, Huiying ;
Ma, Shuangyu ;
Feng, Chong ;
Huang, Yongye ;
Ouyang, Hongsheng ;
Hu, Xiaoxiang ;
Pan, Dengke ;
Li, Ning ;
Wu, Sen .
STEM CELLS, 2015, 33 (11) :3228-3238
[9]   Generation of Induced Pluripotent Stem Cell Lines from Tibetan Miniature Pig [J].
Esteban, Miguel A. ;
Xu, Jianyong ;
Yang, Jiayin ;
Peng, Meixiu ;
Qin, Dajiang ;
Li, Wen ;
Jiang, Zhuoxin ;
Chen, Jiekai ;
Den, Kang ;
Zhong, Mei ;
Cai, Jinglei ;
Lai, Liangxue ;
Pei, Duanqing .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (26) :17634-17640
[10]   Induced Pluripotent Stem Cells from Pigs and Other Ungulate Species: An Alternative to Embryonic Stem Cells? [J].
Ezashi, T. ;
Telugu, B. P. V. L. ;
Roberts, R. M. .
REPRODUCTION IN DOMESTIC ANIMALS, 2012, 47 :92-97