Detection of Viral DNA Sequences in the Cerebrospinal Fluid of Patients With Multiple Sclerosis

被引:39
作者
Mancuso, Roberta [1 ]
Hernis, Ambra [1 ]
Cavarretta, Rosella [2 ]
Caputo, Domenico [2 ]
Calabrese, Elena [3 ]
Nemni, Raffaello [3 ,4 ]
Ferrante, Pasquale [5 ]
Delbue, Serena [5 ]
Clerici, Mario [1 ,4 ]
机构
[1] IRCCS S Maria Nascente, Don C Gnocchi Fdn, Lab Mol Med & Biotechnol, I-20148 Milan, Italy
[2] IRCCS S Maria Nascente, Don C Gnocchi Fdn, Multiple Sclerosis Unit, I-20148 Milan, Italy
[3] IRCCS S Maria Nascente, Don C Gnocchi Fdn, Dept Neurol, I-20148 Milan, Italy
[4] Univ Milan, Dept Biomed Sci & Technol, I-20133 Milan, Italy
[5] Univ Milan, Dept Publ Hlth, I-20133 Milan, Italy
关键词
multiple sclerosis; cerebrospinal fluid; JCV; VZV; HHV-6; EBV; PROGRESSIVE MULTIFOCAL LEUKOENCEPHALOPATHY; JC VIRUS-DNA; EPSTEIN-BARR-VIRUS; VARICELLA-ZOSTER-VIRUS; NORMAL BRAIN-TISSUE; HUMAN-HERPESVIRUS; 6; NATALIZUMAB; REACTIVATION; INFECTION; DISEASE;
D O I
10.1002/jmv.21764
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The role of viruses in the pathogenesis of multiple sclerosis (MS) is a subject of heated debate. The presence of six different neurotropic viruses was sought, including JC virus (JCV), varicella zoster virus (VZV), human herpesvirus 6 (HHV-6), and Epstein-Barr virus (EBV), in cerebrospinal fluid (CSF) samples collected from 51 patients with MS and 30 patients with other neurological diseases. Cell-free or cell-associated viral DNA in CSF samples was detected by real-time PCR, and viral loads were determined Magnetic resonance imaging (MRI) examinations were also performed to look for active lesions. Cell-associated JCV DNA was detected in 3 of the 51 patients with MS and in 2 of the 30 patients with other neurological disease Cell-free JCV DNA was detected in one additional patient with MS. Cellfree VZV DNA was detected in one patient without MS, cell-free HHV-6 was detected in one patient with MS, and cell-free EBV was detected in one patient with MS. All other study patients had no detectable viral DNA in CSF samples and no double infections were found. The small percentage of patients with detectable viral DNA in CSF samples was comparable between patients with MS and those with other neurological disease, and presence of viral DNA was not a predictor of brain lesions. Additional observations suggest that cell trafficking from the periphery, rather than leakage through the blood brain barrier, results in the transport of viruses to the CNS, where local immunosurveillance can control viral replication in immunocompetent individuals. J. Med. Virol. 82:1051-1057, 2010. (C) 2010 Wiley-Liss, Inc
引用
收藏
页码:1051 / 1057
页数:7
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