Application of response surface methodology to cell immobilization for the production of palatinose

被引：56

作者：

Mundra, Piyushkumar ^{[1
]}

Desai, Kiran ^{[1
]}

Lele, S. S. ^{[1
]}

机构：

[1] Inst Chem Technol, Dept Food Engn & Technol, Bombay 400019, Maharashtra, India

来源：

BIORESOURCE TECHNOLOGY | 2007年 / 98卷 / 15期

关键词：

response surface method; cell entrapment; immobilization parameter optimization; palatinose; Erwinia rhopontici;

D O I：

10.1016/j.biortech.2006.09.046

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Response surface methodology (RSM), based on multivariate non-linear model, was applied to study the interactions and optimization of the immobilization parameters for cell entrapment, namely alginate concentration, cell loading and bead diameter using Erwinia rhapontici NCPPB 1578 that produced palatinose. ANOVA analysis and statistical parameters calculations showed that RSM could be used effectively to model and improve a complex system like cell immobilization. Palatinose yield was increased by 40%. The maximum yield of 140 mg/ml was achieved in a batch of I h at alginate concentration of 5% w/v, cell loading of g l(-1) and 2.25 turn bead diameter. Thus, the E. rhapontici NCPPB 1578 immobilization in alginate bead and subsequent palatinose yield was successfully improved by application of RSM technique. (C) 2006 Elsevier Ltd. All rights reserved.

引用

页码：2892 / 2896

页数：5

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