Beneficial Effects of Melatonin on the In Vitro Maturation of Sheep Oocytes and Its Relation to Melatonin Receptors

被引：90

作者：

Tian, Xiuzhi ^{[1
]}

Wang, Feng ^{[1
]}

Zhang, Lu ^{[1
]}

He, Changjiu ^{[1
]}

Ji, Pengyun ^{[1
]}

Wang, Jing ^{[1
]}

Zhang, Zhenzhen ^{[1
]}

Lv, Dongying ^{[1
]}

Abulizi, Wusiman ^{[2
]}

Wang, Xuguang ^{[2
]}

Lian, Zhengxing ^{[1
]}

Liu, Guoshi ^{[1
]}

机构：

[1] China Agr Univ, Coll Anim Sci & Technol, Beijing Key Lab Anim Genet Improvement,Minist Agr, Natl Engn Lab Anim Breeding,Key Lab Anim Genet &, Beijing 100193, Peoples R China

[2] Xinjiang Agr Univ, Coll Anim Sci, Urumqi 830052, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2017年 / 18卷 / 04期

关键词：

melatonin; oocyte; meioctic maturation; MT1/MT2; sheep; PHOSPHODIESTERASE ISOENZYME INHIBITORS; HYALURONIC-ACID SYNTHESIS; ACTIVATED-PROTEIN-KINASE; OVARIAN GRANULOSA-CELLS; MOUSE 2-CELL EMBRYOS; LUTEINIZING-HORMONE; MEIOTIC MATURATION; PORCINE OOCYTE; BOVINE OOCYTES; CUMULUS EXPANSION;

D O I：

10.3390/ijms18040834

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(1) Background: The binding sites of melatonin, as a multifunctional molecule, have been identified in human, porcine, and bovine samples. However, the binding sites and mechanisms of melatonin have not been reported in sheep; (2) Methods: Cumulus oocyte complexes (COCs) were cultured in TCM-199 supplemented with melatonin at concentrations of 0, 10(-3), 10(-5),10(-7), 10(-9), and 10(-11) M. Melatonin receptors (MT1 and MT2) were evaluated via immunofluorescence and Western blot. The effects of melatonin on cumulus cell expansion, nuclear maturation, embryo development, and related gene (GDF9, DNMT1, PTX3, HAS2, and EGFR) expression were investigated. The level of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) were evaluated in oocytes and cumulus, respectively; (3) Results: Both MT1 and MT2 were expressed in oocytes, cumulus cells, and granulosa cells. Melatonin with a concentration of 10-7 M significantly enhanced the rates of nuclear maturation, cumulus cells expansion, cleavage, and blastocyst. Melatonin enhanced the expression of BMP15 in oocytes and of PTX3, HAS2, and EGFR in cumulus cells. Melatonin decreased the cAMP level of oocytes but enhanced the cGMP level in oocytes and cumulus cells; (4) Conclusion: The higher presence of MT1 in GV cumulus cells and the beneficial effects of melatonin indicated that its roles in regulating sheep oocyte maturation may be mediated mainly by the MT1 receptor.

引用

页数：15

共 60 条

[1] Melatonin has dose-dependent effects on folliculogenesis, oocyte maturation capacity and steroidogenesis [J].