Covalent Monofunctionalization of Peptide-Coated Quantum Dots for Single-Molecule Assays

被引:74
作者
Clarke, Samuel [1 ]
Pinaud, Fabien [1 ]
Beutel, Oliver [2 ]
You, Changjiang [2 ]
Piehler, Jacob [2 ]
Dahan, Maxime [1 ]
机构
[1] Univ Paris 06, Ecole Normale Super, Dept Phys & Biol, Lab Kastler Brossel,CNRS,UMR 8552, F-75005 Paris, France
[2] Univ Osnabruck, Fachbereich Biol, D-49076 Osnabruck, Germany
关键词
Quantum dots; single molecules; particle tracking; cell imaging; fluorescent probes; SEMICONDUCTOR NANOCRYSTALS; LIVE CELLS; PROTEINS; TRACKING; SIZE; NANOPARTICLES; MONOVALENT; RECEPTORS; STABILITY; MEMBRANE;
D O I
10.1021/nl100825n
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Fluorescent probes For biological imaging of single molecules (SM) have many stringent design requirements. In the case of quantum dot (QD) probes, it remains a challenge to control their functional properties with high precision. Here, we describe the simple preparation of QDs with reduced size and monovalency. Our approach combines a peptide surface coating, stable covalent conjugation of targeting units and purification by gel electrophoresis. We precisely characterize these probes by ensemble and SM techniques and apply them to tracking individual proteins in living cells.
引用
收藏
页码:2147 / 2154
页数:8
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