Application of Mass Spectrometry in Analysis of Non-Enzymatic Glycation Proteins in Diabetic Blood

Diabetes mellitus is a metabolic disorder disease characterized by hyperglycemia. Long-term physiological environment of hyperglycemia will aggravate the degree of non-enzymatic glycation proteins. Because the abnormal changes of non-enzymatic process will cause a series of pathological changes, non-enzymatic glycation proteins have attracted researchers' attention. Mass spectrometry is an indispensable and powerful analytical tool for qualitative and quantitative analysis of proteins due to its ultra-high sensitivity, excellent detection limit, and multi-component simultaneous analysis, and has been applied to the identification and qualification of non-enzymatic glycation protein. Herein, the principle of non-enzymatic proteins and the research progress of mass spectrometry in the identification and analysis of glycated plasma proteins and hemoglobin in blood were reviewed, and the development trend of this field was prospected.