Effects of the acid polysaccharide fraction isolated from a cultivated Cordyceps sinensis on macrophages in vitro

被引：179
作者：
Chen, Weixia
Zhang, Weiyun [1 ]
Shen, Wenbin
Wang, Kongcheng
机构：
[1] Nanjing Univ, Jiangsu Key Lab Mol Med, Sch Med, Nanjing 210093, Peoples R China
来源：
CELLULAR IMMUNOLOGY | 2010年 / 262卷 / 01期
关键词：
Cordyceps sinensis (Cs); Macrophage; Phagocytosis; NO; iNOS; NF-kappa B; TUMOR-BEARING MICE; EXOPOLYSACCHARIDE FRACTION; FUNGUS; MYCELIA; EXTRACT; NITRITE; OXIDE; EPSF;
D O I：
10.1016/j.cellimm.2010.01.001
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The acid polysaccharide fraction (APSF) extracted from the mycelia of cultivated Cordyceps sinensis is water-soluble polysaccharide. In this study we evaluated the modulating effects of APSF on murine macrophage cell line RAW264.7. Phagocytotic assay by neutral red and FITC-dextran internalization showed that APSF stimulated the phagocytosis of macrophages. The nitrite levels in the culture Supernatant determined using Griess reagent revealed the elevation of NO production after treatment with APSF. RT-PCR and immunocytochemistry assay indicated that APSF promoted both the mRNA and protein expressions of inducible nitric oxide synthase (iNOS). Furthermore, Western blotting demonstrated that NF-kappa B levels in nucleuses increased after APSF treatment, suggesting that APSF probably stimulated macrophage activities by activating the I kappa B-NF-kappa B pathway. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码：69 / 74
页数：6
相关论文
共 20 条
[1]   Antitumor sterols from the mycelia of Cordyceps sinensis [J].
Bok, JW ;
Lermer, L ;
Chilton, J ;
Klingeman, HG ;
Towers, GHN .
PHYTOCHEMISTRY, 1999, 51 (07) :891-898
[2]  
Chang YH, 2009, IN VIVO, V23, P755
[3]   Morphological and genetic characterization of a cultivated Cordyceps sinensis fungus and its polysaccharide component possessing antioxidant property in H22 tumor-bearing mice [J].
Chen, JP ;
Zhang, WY ;
Lu, TT ;
Li, J ;
Zheng, Y ;
Kong, LD .
LIFE SCIENCES, 2006, 78 (23) :2742-2748
[4]   Studies of macrophage immuno-modulating activity of polysaccharides isolated from Paecilomyces tenuipes [J].
Chen, Xiaoming ;
Lu, Jianxin ;
Zhang, Yangde ;
He, Jiantai ;
Guo, Xunzhi ;
Tian, Gengyuan ;
Jin, Liqin .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2008, 43 (03) :252-256
[5]   Nitrite oxide and inducible nitric oxide synthase were regulated by polysaccharides isolated from Glycyrrhiza uralensis Fisch [J].
Cheng, Anwei ;
Wan, Fachun ;
Jin, Zhengyu ;
Wang, Jiaqi ;
Xu, Xueming .
JOURNAL OF ETHNOPHARMACOLOGY, 2008, 118 (01) :59-64
[6]   Alternative activation of macrophages [J].
Gordon, S .
NATURE REVIEWS IMMUNOLOGY, 2003, 3 (01) :23-35
[7]   ANALYSIS OF NITRATE, NITRITE, AND [N-15]-LABELED NITRATE IN BIOLOGICAL-FLUIDS [J].
GREEN, LC ;
WAGNER, DA ;
GLOGOWSKI, J ;
SKIPPER, PL ;
WISHNOK, JS ;
TANNENBAUM, SR .
ANALYTICAL BIOCHEMISTRY, 1982, 126 (01) :131-138
[8]   Upregulation of costimulatory molecules induced by lipopolysaccharide and double-stranded RNA occurs by Trif-dependent and Trif-independent pathways [J].
Hoebe, K ;
Janssen, EM ;
Kim, SO ;
Alexopoulou, L ;
Flavell, RA ;
Han, JH ;
Beutler, B .
NATURE IMMUNOLOGY, 2003, 4 (12) :1223-1229
[9]   Antiaging Effect of Cordyceps sinensis Extract [J].
Ji, Deng-Bo ;
Ye, Jia ;
Li, Chang-Ling ;
Wang, Yu-Hwa ;
Zhao, Jiong ;
Cai, Shao-Qing .
PHYTOTHERAPY RESEARCH, 2009, 23 (01) :116-122
[10]   TUMOR-NECROSIS-FACTOR ALPHA-INDUCED PHOSPHORYLATION OF I-KAPPA-B-ALPHA IS A SIGNAL FOR ITS DEGRADATION BUT NOT DISSOCIATION FROM NF-KAPPA-B [J].
MIYAMOTO, S ;
MAKI, M ;
SCHMITT, MJ ;
HATANAKA, M ;
VERMA, IM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (26) :12740-12744
12