A secreted WY-domain-containing protein present in European isolates of the oomycete Plasmopara viticola induces cell death in grapevine and tobacco species

被引：14

作者：

Combier, Maud ^{[1
]}

Evangelisti, Edouard ^{[2
]}

Piron, Marie-Christine ^{[1
]}

Rengel, David ^{[3
]}

Legrand, Ludovic ^{[3
]}

Shenhav, Liron ^{[2
]}

Bouchez, Olivier ^{[4
]}

Schornack, Sebastian ^{[2
]}

Mestre, Pere ^{[1
]}

机构：

[1] Univ Strasbourg, INRA, SVQV, Colmar, France

[2] Univ Cambridge, Sainsbury Lab SLCU, Cambridge, England

[3] Univ Toulouse, INRA, CNRS, LIPM, Castanet Tolosan, France

[4] Genotoul, INRA, US GeT PlaGe 1426, Castanet Tolosan, France

来源：

PLOS ONE | 2019年 / 14卷 / 07期

关键词：

CANDIDATE RXLR EFFECTOR; PHYTOPHTHORA-INFESTANS; DISEASE-RESISTANCE; SUBCELLULAR-LOCALIZATION; GENE-EXPRESSION; PLANT IMMUNITY; HOST; PREDICTION; TRANSIENT; AVR3A;

D O I：

10.1371/journal.pone.0220184

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Plasmopara viticola is a biotrophic oomycete pathogen causing grapevine downy mildew. We characterized the repertoire of P. viticola effector proteins which may be translocated into plants to support the disease. We found several secreted proteins that contain canonical dEER motifs and conserved WY-domains but lack the characteristic RXLR motif reported previously from oomycete effectors. We cloned four candidates and showed that one of them, Pv33, induces plant cell death in grapevine and Nicotiana species. This activity is dependent on the nuclear localization of the protein. Sequence similar effectors were present in seven European, but in none of the tested American isolates. Together our work contributes a new type of conserved P. viticola effector candidates.

引用

页数：22

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