Refolding of mis-folded recombinant cytochrome c3 with strong cation exchange chromatography

被引：1

作者：

Shen, Yehua ^{[1
]}

Takayama, Yuki

Wei, Yinmao

Harada, Erisa

Nakatani, Eiichi

Akutsu, Hideo

机构：

[1] NW Univ Xian, Inst Drug Discovery, Dept Chem, Xian 710069, Peoples R China

[2] Osaka Univ, Inst Prot Res, Osaka, Japan

[3] Japan Sci & Technol Agcy, Inst Bioinformat Res & Dev, Tokyo, Japan

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2007年 / 30卷 / 5-8期

关键词：

Shewanella oneidensis; recombinant cytochrome c(3); refolding; strong cation exchange chromatography;

D O I：

10.1080/10826070601128410

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Mis-folded recombinant cytochrome c(3) (rcyt c(3)) formed in the purification process was refolded with strong cation exchange chromatography, with tri-gradient of salt, urea, and pH in the mobile phase. The optimal concentration of urea was found to be 6.0 mol/L in mobile phase to refold the mis-folded rcyt c(3) under the experimental conditions. The recovery of the correctly refolded rcyt c(3) was calculated to be 57.0%.

引用

页码：1051 / 1059

页数：9

共 8 条

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